CELL IMAGING AND HISTOPATHOLOGY

细胞成像和组织病理学

• 批准号: 8378391
• 负责人: BARBARA RANSCHT
• 金额: $ 26.86万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-21 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8378391
• 关键词: Address; Algorithms; Apoptosis; Budgets; Cancer Center; Cancer Center Support Grant; Cell Communication; Cells; Cellular Structures; Communication; Computer software; Computers and Advanced Instrumentation; Consult; Consultations; Data; Data Analyses; Dependence; Detection; Development; Disease; Dissection; Documentation; Electron Microscope; Electron Microscopy; Electrons; Endocytosis; Environment; Equipment; Event; Exocytosis; Experimental Genetics; Experimental Models; Fluorescence Resonance Energy Transfer; Generations; Genes; Genetic; Genetic Models; Histology; Histopathology; Histopathology Facility; Housing; Human; Image; Image Analysis; Imaging Device; Imaging Techniques; Immunohistochemistry; Institutes; Internet; Investigation; Label; Lasers; Life; Location; Malignant Neoplasms; Microscope; Microscopic; Mission; Molecular; Molecular Probes; Monitor; Movement; Mus; Names; Normal Cell; Organelles; Pathologist; Patients; Persons; Population; Preparation; Process; Proteins; Protocols documentation; Qualifying; Research; Research Personnel; Research Support; Resolution; Resource Sharing; Resources; Scanning; Science; Services; Signal Transduction; Slide; Specimen; Staining method; Stains; System; Techniques; Technology; Time; Tissue Microarray; Tissue Sample; Tissues; Training; Training Support; Work; cancer cell; carcinogenesis; cell motility; cellular imaging; cost effective; data acquisition; data sharing; design; digital; drug modification; experience; fluorescence microscope; image archival system; improved; instrument; instrumentation; investigator training; laser capture microdissection; member; molecular imaging; molecular pathology; molecular/cellular imaging; mouse model; multi-photon; receptor; research study; response; tissue culture; tissue processing; tool; transmission process

Observations of molecular changes in patient tissues or caused by genetic or experimental manipulations in mice and cancer cells in culture critically depend on histological and immunohistological analyses in cells and tissues through advanced imaging techniques. The mission of the Cell Imaging and Histopathology Shared Resource is to provide state-of-the-art imaging and histopathology tools, training, and services to Cancer Center researchers. The Shared Resource is divided into two adjacent Facilities: 1) The Cell /mag/ng facility houses four confocal microscopes and five additional fluorescence microscopes. All the microscopes are equipped with advanced digital cameras and image analysis software. Available systems include laser scanning confocal microscopes with single and multi-photon lasers, as well as a spinning disk confocal microscope for extended live cell imaging. Time-lapse imaging systems are available to study cell motility, proliferation, and differentiation, with FRET and TIRF systems to image intracellular dynamic interactions. A full range of wide-field imaging approaches are also supported. The facility transmission electron microscope service provides analysis of cell and tissue ultrastructure. Facility staff maintains the imaging equipment and provides investigator training and support for imaging and image analysis, as well as imaging service, and active development of new imaging tools. 2) The Histopathology facility offers the preparation, sectioning, staining and analysis of tissue samples for histological and histopathological analyses and probe-specific detection of cellular and molecular changes in tissue sections. For analysis of identified cells or cell groups within tissue sections, the facility offers Laser Capture Microdissection services. An automated slide scanning system affords efficient histological data acquisition, image archiving, analysis and quantitation. A staff pathologist with expertise in both human and mouse specimens provides consultation, as well as assistance with tissue microarrays. The Facility staff provides consultation, user training, development and dissemination of imaging-, histology- and immunohistochemical protocols, and assistance with instrumentation for efficient histology data documentation, analysis and storage. The Shared Resource supported the studies of 35 Cancer Center Members in the past year, and is essential to advance the understanding of molecular and cellular events leading to cancer development and spreading. Overall, $185,234 in CCSG support is requested in the first year, representing 18.4% of the total projected annual operating budget.

观察患者组织中的分子变化或由遗传或实验操作引起的分子变化， 小鼠和癌细胞的培养关键取决于细胞的组织学和免疫组织学分析 和组织的图像。细胞成像和组织学的使命 共享资源旨在提供最先进的成像和组织病理学工具、培训和服务， 癌症中心的研究人员。共享资源分为两个相邻的设施： 1)Cell /mag/ng设施拥有四台共聚焦显微镜和五台额外的荧光显微镜。 所有的显微镜都配备了先进的数码相机和图像分析软件。可用 系统包括具有单光子和多光子激光器的激光扫描共焦显微镜，以及 用于扩展活细胞成像的旋转圆盘共聚焦显微镜。时间推移成像系统是可用的 研究细胞运动，增殖和分化，与FRET和TIRF系统成像细胞内 动态互动还支持全方位的宽视场成像方法。该设施 透射电子显微镜服务提供细胞和组织超微结构分析。设施工作人员 维护成像设备，并为研究者提供成像和影像学培训和支持 分析，以及成像服务，并积极开发新的成像工具。 2)组织学设施提供组织样本的制备、切片、染色和分析， 组织学和组织病理学分析以及细胞和分子变化的探针特异性检测 组织切片。为了分析组织切片中已识别的细胞或细胞群，该设施提供激光 捕获显微切割服务。自动载玻片扫描系统提供有效的组织学数据 采集、图像存档、分析和定量。一位在人类和 老鼠标本提供咨询，以及协助组织微阵列。设施工作人员 提供咨询，用户培训，开发和传播成像，组织学， 免疫组化方案，并协助仪器有效的组织学数据 记录、分析和储存。共享资源支持35癌症中心的研究 成员在过去的一年中，是必不可少的，以促进对分子和细胞事件的理解 导致癌症发展和扩散。总体而言，在第一阶段， 2011年，占预计年度业务预算总额的18.4%。