Core--Xenograft Facility

通过开发和维持几个连续可移植的GBM肿瘤系，每一个都已经或将被表征为GBM标志性病变，Xenograft Core将为SPORE研究人员提供强大的动物模型，再现人类表型中所见的侵袭性。连续传代的异种移植物提供了比作为异种移植物生长的已建立的细胞系更具有临床相关性的模型。这一结论对神经肿瘤学研究有许多意义，但对实验疗法的测试可能是最重要的。我们的论点是，连续移植的GBM异种移植物的抗肿瘤治疗测试结果将显示出与人类临床试验结果的一致性。

Through the development and maintenance of several serially transplantable GBM tumor lines, each of which has been or will be characterized for GBM signature lesions, the Xenograft Core will provide to SPORE investigators robust animal models that recapitulate the invasiveness seen in the human phenotype. The serially passaged xenografts provide a more clinically relevant model than established cell lines grown as xenografts. This determination has many implications for neuro-oncology research, but is likely to be of greatest importance to the testing of experimental therapeutics. It is our contention that results from testing anti-tumor therapeutics with serially-transplanted GBM xenografts will show improved consistency with clinical trial results in humans. Specifically, the Xenograft Core will: 1: Initiate, passage, and archive xenograft tumors derived from Mayo glioma patients. 2: Provide detailed molecular characterization of each xenograft upon establishment and monitor for retention of characteristics. 3: Collect, process, and distribute within and outside Mayo, paraffin-embedded xenograft tumor tissues, serum/plasma, DNA, RNA, and protein. 4: Coordinate and conduct all in vivo therapeutics testing, including NMR image analysis of intracranial xenografts as needed to assess tumor response to therapy. 5: Maintain up-to-date records on all xenograft tissues and related biospecimens. All specimens will be collected and processed under tight quality control, and will be distributed to SPORE researchers or banked for future SPORE research projects. The SPORE Pathology Core will assure that specimens are properly fixed, stained, and histologically evaluated. All activities will be tracked in the Biostatistics Core using a sophisticated database essential to the SPORE. This database merges the activities at Mayo, and allows integration with clinical and other data collected in research projects.