PRE-CLINICAL ANIMAL CORE

临床前动物核心

• 批准号: 8514330
• 负责人: Charles David James
• 金额: $ 16.49万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8514330
• 关键词: Abnormal Cell; Address; Allografting; Animal Euthanasia; Animal Experimentation; Animal Model; Animals; Apoptotic; Archives; Biodistribution; Biological Assay; Biological Markers; Bioluminescence; Blood specimen; Brain; Brain Neoplasms; CD-I; Cell Count; Cell Line; Cells; Collaborations; Data; Data Quality; Development; Engraftment; Euthanasia; Frequencies; Grant; Growth; Human; Immunocompetent; Implant; In Vitro; Instruction; Intraperitoneal Injections; Investigation; Investigational Therapies; Ionizing radiation; Ki-67 Antigen; Length; Life; Luciferases; Malignant - descriptor; Methods; Modeling; Molecular; Monitor; Mus; Neurologic Symptoms; Nude Mice; Operative Surgical Procedures; Oral; Outcome; Pathology; Patients; Pharmaceutical Preparations; Phase; Principal Investigator; Procedures; Process; Protocols documentation; Rattus; Records; Reproduction spores; Research; Research Personnel; Resected; Resources; Rodent; Rodent Model; Source; Specimen; Staining method; Stains; Stem cells; Surrogate Markers; Survival Analysis; Testing; Therapeutic; Therapeutic Effect; Tissue and Organ Harvesting; Tissues; Toxic effect; Training; Tumor Burden; Tumor Cell Line; Tumor Tissue; Vascularization; Xenograft procedure; assay development; base; bioluminescence imaging; caspase-3; design; experience; in vivo; irradiation; mouse model; neoplastic cell; optical imaging; pre-clinical; pre-clinical therapy; research study; response; subcutaneous; therapeutic target; tumor; tumor growth; tumorigenic

Because of the translational requirement of SPORE research, it is essential that SPORE investigators have access to and assistance with animal models for therapeutic hypothesis testing. The UCSF Brain Tumor SPORE Animal Core addresses this need by using 3 types of rodent intracranial engraftment models, based on cell of origin: 1) human tumor cells; 2) chemically induced rodent brain tumor cell lines; and 3) tumor cells derived from genetically modified mouse models. Tumor cells are implanted in the brains of immunodeficient, and/or immunocompetent hosts, with therapeutic effect determined by bioluminescence monitoring of tumor growth, animal subject survival analysis, and immunohistochemical analysis of tumor biologic response indicators, especially proliferation and apoptotic response. In addition, the Core also conducts studies to assess therapeutic toxicity and biodistribution. These studies typically involve organ and tissue harvests at pre-determined timepoints, with specimens examined for drug content, and/or indication of abnormal pathology, and/or abnormal cell counts when blood samples are obtained. Finally, the Core serves as a source of tumor tissues, resulting from engraftment procedures, for biomarker investigation and assay development, and for in vitro investigation in instances involving the transfer of viable tissues or cells. These activities are carried out in association with the following specific aims: Aim 1: Propagate, analyze (histopathological and molecular), archive, and maintain up-to-date records on all tumor cell sources and tissues used in support of SPORE animal model research. Aim 2: Advise and assist all rodent model therapeutics testing, including optical imaging, survival benefit analysis, toxicity assessment, and molecular analyses of tumors for response to therapy. Aim 3: In association with the Tissue Core, utilize human xenograft tumor tissues to facilitate the development of immunohistochemical and FISH assays that can be applied to the investigation of biologic response indicators, therapeutic targets, and surrogate markers in patient tumors. Aim 4: Process, and distribute, within and outside of UCSF, xenograft tumor tissues and cell lines, as well as extracts from each, so as to promote intra- and inter-SPORE collaborations, as well as to support brain tumor research in general, through utilization of renewable tumor cell resources. RELEVANCE (See instructions): Animal model research is a required part of testing investigational therapies prior to their being used to treat brain tumor patients. As such, and given the translational orientation of SPORE research, there is an ongoing need of SPORE investigators to perform animal model studies. Such studies are made more efficient by having specialized staff with animal model research expertise, and who are readily available to assist in the design and implementation of SPORE animal model research.

由于《孢子》研究的转译要求，《孢子》研究者必须具备