Identifying potential synergy between pleurotin and bacteriophages towards tackling multi- resistant infections.

确定侧耳素和噬菌体之间在应对多重耐药感染方面的潜在协同作用。

• 批准号: 2391871
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2391871
• 关键词: Identifying; potential; synergy; between; pleurotin

Multidrug-resistant organisms pose a major healthcare challenge. The current limitations on antibiotic discovery, have necessitated the search for novel discovery methods and sources as well as non-antibiotic alternatives. The basidiomycete-derived, secondary metabolite pleurotin and its congeners could be a proponent of the former, as these have been shown to be effective against gram-positive bacteria, while bacteriophages (phages) could be the ultimate non-antibiotic alternative. Phages can target with great specificity (resistant) bacterial pathogens. A very promising application of bacteriophage therapy is phage-antibiotic combination (PAC) therapy, where cocktails of phage and conventional antibiotic are employed against problematic bacterial strains. In this project, we wish to examine the landscape of phage and antibiotic combination therapy research, to find out if phage-antibiotic synergy (PAS) can be achieved, which could inhibit bacterial growth, increase biofilm clearance and counteract the emergence of bacterial resistance. Along this effort, the basidiomycete-derived pleurotin and its congeners will be explored as ways to discover novel secondary metabolites of interest. An examination of the combination of pleurotin and bacteriophages targeting the gram-positive Staphylococcus aureus infections will be analysed to reveal efficiency and cytotoxicity in human cells.

耐多药的生物构成了一个重大的医疗挑战。目前抗生素发现的局限性，使得寻找新的发现方法和来源以及非抗生素替代品成为必要。担子菌衍生的次级代谢物侧耳素及其同系物可能是前者的支持者，因为它们已被证明对革兰氏阳性菌有效，而噬菌体(噬菌体)可能是最终的非抗生素替代品。噬菌体可以以高度特异性(抗药性)的细菌病原体为靶标。噬菌体疗法的一个非常有前途的应用是噬菌体-抗生素组合(PAC)疗法，即使用噬菌体和常规抗生素的鸡尾酒来对抗有问题的细菌菌株。在本项目中，我们希望考察噬菌体和抗生素联合治疗的研究现状，以确定是否可以实现噬菌体-抗生素协同作用(PAS)，从而抑制细菌生长，增加生物被膜清除，并对抗细菌耐药性的出现。随着这一努力的进行，担子菌衍生的侧耳素及其同系物将被探索为发现感兴趣的新的次生代谢物的方法。针对革兰氏阳性金黄色葡萄球菌感染的胸苷肽和噬菌体组合的检查将被分析，以揭示对人类细胞的效率和细胞毒性。