Expanding Combinatorial Space in Bacterial Libraries for Synthetic Biology Applications

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Although Synthetic and Systems Biology aspire to the design of biological systems,combinatorial approaches remain efficient strategies to rapidly isolate functional designsfrom a vast number of possible designs. High-quality libraries are therefore essential andtheir assembly hinges on multiple practical and technical issues: (1) robustness of synthesis,(2) stability and (3) sampling of the sequence space. Having recently developed a novelapproach to establish large multi-component in vivo libraries, we want to expand theapproach towards studying the fitness of synthetic phages and microcompartmentencapsulation of biological processes - by improving (1) and developing novel assays toconfirm (3).