Expanding Combinatorial Space in Bacterial Libraries for Synthetic Biology Applications

扩大细菌文库的组合空间以用于合成生物学应用

• 批准号: 2397286
• 金额: --
• 依托单位: Birkbeck, University of London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2397286
• 关键词: Expanding; Combinatorial; Space; Bacterial; Libraries

Although Synthetic and Systems Biology aspire to the design of biological systems,combinatorial approaches remain efficient strategies to rapidly isolate functional designsfrom a vast number of possible designs. High-quality libraries are therefore essential andtheir assembly hinges on multiple practical and technical issues: (1) robustness of synthesis,(2) stability and (3) sampling of the sequence space. Having recently developed a novelapproach to establish large multi-component in vivo libraries, we want to expand theapproach towards studying the fitness of synthetic phages and microcompartmentencapsulation of biological processes - by improving (1) and developing novel assays toconfirm (3).

虽然合成和系统生物学渴望生物系统的设计，但组合方法仍然是快速分离功能设计的有效策略。因此，高质量的文库是必不可少的，它们的组装取决于多个实际和技术问题：（1）合成的鲁棒性，（2）稳定性和（3）序列空间的采样。最近开发了一种新的方法来建立大型多组分体内文库，我们希望通过改进（1）和开发新的检测方法来证实（3），将该方法扩展到研究合成药物和生物过程微区室封装的适应性。