Structures of G Protein Signaling Complexes

描述(由申请人提供)： 这些实验旨在确定G蛋白信号系统中膜界面的蛋白质复合体的结构。将以脊椎动物视杆视觉转导通路为模型。通过将特定的探针拴在G蛋白转导蛋白表面的特定位置，将利用荧光共振能量转移和电子顺磁共振光谱来确定与膜的相对距离。这些距离将测试蛋白质的非活性构象以及激活的构象，并在下游效应器存在的情况下进行测试。

DESCRIPTION (provided by applicant): The proposed experiments are designed to determine the structures of the protein complexes at the membrane interface in G protein signaling systems. The well-characterized vertebrate rod vision transduction pathway will be used as a model. By tethering specialized probes at specific sites on the surface of the G protein transducin, fluorescence resonance energy transfer and electron paramagnetic resonance spectroscopy will be utilized to determine the relative distance to the membrane. These distances will be tested for the inactive conformation of the protein, as well as with the activated conformation, and in the presence of downstream effectors.