Structures of G Protein Signaling Complexes

描述（由申请人提供）： 所提出的实验旨在确定G蛋白信号系统中膜界面处的蛋白质复合物的结构。充分表征的脊椎动物视杆视觉转导通路将用作模型。通过在G蛋白转导素表面的特定位点拴系专门的探针，将利用荧光共振能量转移和电子顺磁共振光谱来确定与膜的相对距离。将针对蛋白质的非活性构象以及活化构象和存在下游效应物的情况测试这些距离。

DESCRIPTION (provided by applicant): The proposed experiments are designed to determine the structures of the protein complexes at the membrane interface in G protein signaling systems. The well-characterized vertebrate rod vision transduction pathway will be used as a model. By tethering specialized probes at specific sites on the surface of the G protein transducin, fluorescence resonance energy transfer and electron paramagnetic resonance spectroscopy will be utilized to determine the relative distance to the membrane. These distances will be tested for the inactive conformation of the protein, as well as with the activated conformation, and in the presence of downstream effectors.