Characerization of NDR1 in Arabidopsis thaliana

拟南芥中 NDR1 的表征

• 批准号: 6696941
• 负责人: ROBERT B DAY
• 金额: $ 5.05万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6696941
• 关键词: Characerization; NDR1; Arabidopsis; thaliana

DESCRIPTION (provided by applicant): In the 1940s, Flor proposed the now classic gene-for-gene model to explain the molecular basis of a plant's resistance to a bacterial pathogen. In short, this model suggests that in order for a plant to be resistant to a given pathogen, both genes, avr and R, must be present. The simplest explanation for this interaction is the receptor-ligand model, whereby the resistance gene products (R proteins) recognize, either directly or indirectly, the ligands specified by the bacterial avirulence gene products. Recognition of the ligand by the receptor is thought to initiate a signal transduction cascade leading to the activation of a variety of local and systemic defense responses. NDR1 of Arabidopsis is required for the activity of several CC/NBS/LRR resistance genes, and over-expression of NDR1 provides a dose-dependent enhanced bacterial resistance. Computer analyses suggest that NDR 1 may be a GPI-anchored protein, and as such, may be plasma membrane-localized. Localization at the plasma membrane would place NDR1 in intimate contact with the bacteria-plant cell interface. The goal of this project is to utilize a combination of genetic and biochemical approaches to identify the role NDR1 plays in the pathogen-induced defense response in Arabidopsis.

描述（由申请人提供）：20 世纪 40 年代，Flor 提出了现在经典的基因对基因模型来解释植物对细菌病原体抵抗力的分子基础。简而言之，该模型表明，为了使植物能够抵抗特定的病原体，avr 和 R 基因必须同时存在。这种相互作用的最简单的解释是受体-配体模型，其中抗性基因产物（R蛋白）直接或间接地识别由细菌无毒力基因产物指定的配体。受体对配体的识别被认为启动信号转导级联，从而激活多种局部和全身防御反应。拟南芥的 NDR1 是多个 CC/NBS/LRR 抗性基因的活性所必需的，并且 NDR1 的过表达提供了剂量依赖性增强的细菌抗性。计算机分析表明，NDR 1 可能是一种 GPI 锚定蛋白，因此可能位于质膜上。定位在质膜上将使 NDR1 与细菌-植物细胞界面紧密接触。该项目的目标是利用遗传和生化方法相结合来确定 NDR1 在拟南芥病原体诱导的防御反应中所起的作用。