Characerization of NDR1 in Arabidopsis thaliana

拟南芥中 NDR1 的表征

• 批准号: 6843759
• 负责人: Robert Bradley Day
• 金额: $ 5.35万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6843759
• 关键词: Characerization; NDR1; Arabidopsis; thaliana

18. GOALS FOR FELLOWSHIP TRAINING AND CAREER The primary goal of my research while working in the Staskawicz lab is to continue my professional education in the field of plant-microbe interactions. My interest in this field of study has spanned both the biochemical and genetic aspects of plant physiology, from the beneficial association of Rhizobium-legume symbiosis, to my former post-doctoral position and including my present position, where I have developed an interest in plant pathology and disease resistance. I am confident that my time in Dr. Staskawicz's lab will prove fruitful, and that I will have the opportunity to not only sharpen my own skills as a scientist, yet also be able contribute to the research goals of the Staskawicz lab in general. I hope to leave the Staskawicz laboratory and assume a faculty research position at a university where I will have the opportunity to develop my own research program, as well as to train and educate young scientists. -li_'l_N'li 19. NAMEANDDEGREE(SB) rian J. Staskawicz, Ph.D. 20.POSITION/RANK Professor, Plant and Microbial Biology 21. RESEARCH INTERESTS/AREAS Molecular Genetics of Plant Pathogen Interactions t ;[,,1__,1;te_ -"III No] _IoI.?'.. 22. DESCRIPTION In the 1940s, Flor proposed the now classic gene-for-gene model to explain the molecular basis of a plant's resistance to a bacterial pathogen. In short, this model suggests that in order for a plant to be resistant to a given pathogen, both genes, avr and R, must be present. The simplest explanation for this interaction is the receptor- ligand model, whereby the resistance gene products (R proteins) recognize, either directly or indirectly, the ligands specified by the bacterial avirulence gene products. Recognition of the ligand by the receptor is thought to initiate a signal transduction cascade leading to the activation of a variety of local and systemic defense responses. NDR1 of Arabidopsis is required for the activity of several CC/NBS/LRR resistance genes, and over-expression of NDR1 provides a dose-dependent enhanced bacterial resistance. Computer analyses suggest that NDR 1 may be a GPI- anchored protein, and as such, may be plasma membrane-localized. Localization at the plasma membrane would place NDR1 in intimate contact with the bacteria-plant cell interface. The goal of this project is to utilize a combination of genetic and biochemical approaches to identify the role NDR1 plays in the pathogen-induced defense response in Arabidopsis. PHS 416-1 (Rev. 12/98) Form Page 2 BB cc Individual NRSA Application Table of Contents ========================================Section End===========================================

18. 我在Staskawicz实验室工作期间的主要研究目标是继续我在植物-微生物相互作用领域的专业教育。我对这一研究领域的兴趣跨越了植物生理学的生化和遗传方面，从根瘤菌与豆科植物共生的有益联系，到我以前的博士后职位，包括我现在的职位，我对植物病理学和抗病性产生了兴趣。我相信我在Staskawicz博士的实验室的时间将证明是富有成效的，我将有机会不仅提高我自己的技能作为一个科学家，而且也能够为Staskawicz实验室的研究目标做出贡献。我希望离开Staskawicz实验室，在一所大学担任教职研究职位，在那里我将有机会发展自己的研究计划，并培养和教育年轻的科学家。-li_ l_N将会19。姓名和学位（SB） rian J. Staskawicz，博士植物与微生物学教授植物病原相互作用的分子遗传学研究[j]；[j]；te_ -“（III No）……”22. 在20世纪40年代，Flor提出了现在经典的基因对基因模型来解释植物抵抗细菌病原体的分子基础。简而言之，该模型表明，为了使植物对给定的病原体具有抗性，必须同时存在avr和R两个基因。对这种相互作用最简单的解释是受体-配体模型，即抗性基因产物（R蛋白）直接或间接地识别细菌无毒基因产物指定的配体。受体对配体的识别被认为启动信号转导级联，导致各种局部和全身防御反应的激活。拟南芥的几种CC/NBS/LRR抗性基因的活性都需要NDR1，并且NDR1的过表达提供了剂量依赖性的细菌抗性增强。计算机分析表明ndr1可能是一种GPI锚定蛋白，因此可能是质膜定位的。在质膜上定位将使NDR1与细菌-植物细胞界面密切接触。本项目的目标是利用遗传和生化方法的结合来确定NDR1在拟南芥病原体诱导的防御反应中所起的作用。小灵通416 - 1(启12/98)表单页面2 BB cc个人NRSA应用程序目录 ======================================== 节结束 ===========================================