Developing New Skills for Mouse Neurogenetics Research

开发小鼠神经遗传学研究的新技能

• 批准号: 6747702
• 负责人: WAYNE N. FRANKEL
• 金额: $ 13.45万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-15 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6747702
• 关键词: animal care; behavioral genetics; clinical research; genetic models; genetic screening; genotype; health science research support; high throughput technology; laboratory mouse; model design /development; mutant; neurogenetics; neuropsychology; phenotype; training; travel

DESCRIPTION (provided by applicant): Developments in mouse genetics and genomics have put researchers in a strong position to detect, study and derive insight from mouse models for human disorders, including those for neurological and behavioral defects. Specifically, advances in gene discovery led to the launch of NIH initiatives in phenotype assay development and in large-scale mutagenesis centers with the goal to detect, produce, characterize and make available new animal models to the research community. The Neuroscience Mutagenesis Facility (NMF) at The Jackson Laboratory (TJL) is one of three Centers which has just started to produce mutants using phenotyping assays already developed. A critical component of the NMF is that it must remain cutting edge and in tune with the research community's needs, continually evolving its body of expertise. Conversely, TJL and the NMF have a great deal of expertise in mouse genetics and forward genetic screens to offer to visiting scientists and trainees. Towards these ends, in one track of our proposal, established investigators work in the NMF for a period of one month to one year as Visiting Investigators, selected for their expertise in an area of neurobehavioral research that either complements the NMF or has the ability to greatly enhance it. In turn, these investigators learn how to apply their approaches to forward genetic screens in mouse, which typically means learning how to evaluate parameters across multiple genotypes, how to detect outliers and how to extract the most amount of specific information from a high-throughput assay. A unique advantage of sponsoring this type of activity here is that the NMF is a centralized facility with most of the screening done "under one roof," thus offering the chance to accumulate, consolidate and potentially to install these training experiences efficiently into our operating procedures. In the second track, advanced undergraduate or predoctoral students come to the NMF to learn the basics of mouse neurogenetics and working with laboratory mice while gaining the sense that their contribution is important to our program. Students come from TJL's longstanding student programs or are identified as exceptional candidates from Visiting Investigators' home groups. TJL's experience with these programs and diverse slate of courses and conferences serve to enhance a student's perspective on their training. Both tracks require development of research plans in advance of the training period, and both have methods in place to evaluate success. We request funds to support the travel and lodging of two Visiting Investigators and two Students at any given time, plus the supplies, animal care resources and faculty salary required to support these activities.

描述（由申请人提供）：小鼠遗传学和基因组学的发展使研究人员处于有利地位，可以检测、研究和从小鼠模型中获得人类疾病的见解，包括神经和行为缺陷。具体来说，基因发现的进步导致NIH启动了表型分析开发和大规模诱变中心的倡议，目标是检测，生产，表征并为研究界提供新的动物模型。杰克逊实验室（TJL）的神经科学诱变设施（NMF）是刚刚开始使用已经开发的表型分析产生突变体的三个中心之一。NMF的一个关键组成部分是，它必须保持前沿，与研究界的需求保持一致，不断发展其专业知识体系。相反，TJL和NMF在小鼠遗传学和遗传筛选方面有大量的专业知识，可以为来访的科学家和学员提供。为了达到这些目的，在我们提议的一个轨道上，建立研究者在NMF工作一个月到一年的访问研究者，选择他们在神经行为研究领域的专业知识，要么补充NMF，要么有能力极大地增强它。反过来，这些研究人员学习如何将他们的方法应用于小鼠的遗传筛选，这通常意味着学习如何评估多个基因型的参数，如何检测异常值以及如何从高通量分析中提取最大量的特定信息。赞助这类活动的一个独特优势是，NMF是一个集中的设施，大多数筛选都在“一个屋檐下”完成，因此提供了积累、巩固和潜在地将这些培训经验有效地应用到我们的操作程序中的机会。在第二个轨道上，高级本科生或博士生来到NMF学习小鼠神经遗传学的基础知识，并与实验室小鼠一起工作，同时获得他们对我们项目的重要贡献的感觉。学生来自TJL的长期学生项目或被确定为来自访问研究者家庭小组的特殊候选人。TJL在这些项目上的经验以及多样化的课程和会议有助于提高学生对他们的培训的看法。这两个方向都需要在培训期之前制定研究计划，并且都有适当的方法来评估成功。我们要求资金支持两名访问调查员和两名学生在任何给定时间的旅行和住宿，以及支持这些活动所需的用品，动物护理资源和教师工资。