Herbal Angiogenesis Stimulator for Chronic Wound Healing

用于慢性伤口愈合的草药血管生成刺激剂

• 批准号: 6736871
• 负责人: HUI ZHAO
• 金额: $ 9.96万
• 依托单位: SUNNY BIODISCOVERY, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6736871
• 关键词: SCID mouse; X ray crystallography; alternative medicine; angiogenesis factor; angiosperms; chemical structure; clinical research; drug design /synthesis /production; fibroblast growth factor; histochemistry /cytochemistry; human tissue; laboratory rat; mass spectrometry; medicinal plants; nonsurgical revascularization; nuclear magnetic resonance spectroscopy; plant extracts; skin transplantation; wound; wound healing; xenotransplantation

DESCRIPTION (provided by applicant): Chronic wounds translate into daily suffering for 7 million elderly people in United States and Europe alone. Modern dressings, as used today, are insufficient for optimal treatment of these wounds because most often they do not include compounds actively promoting wound healing. Stimulation of angiogenesis (wound vascularization) is a key requirement and a logic therapeutic approach to chronic wound healing. However, wound dressings, which include proteinaceous angiostimulators, are disappointing, because these stimulators are highly labile and cannot be maintained in treatment sites at therapeutic levels. Here we propose to test for enhancement of wound healing a small and stable angiostimulatory and fibroblast stimulatory molecule isolated in our laboratory from a Chinese medicinal plant. Using planimetry and advanced histochemistry we will test the effect of this stimulator on the speed and completeness of wound closure and on the quality of the reepithelialized tissue in animal and chimera human skin models. Furthermore, we will determine the chemical structure of this molecule with NMR, mass spectrometry, elemental analysis and crystallography. Taken together, this project aims to provide the proof-of-principle that a small stable angiogenesis/fibroblast stimulator can significantly improve wound healing and lead to a novel, commercially viable therapy for the treatment of chronic wounds.

描述(由申请人提供)： 仅在美国和欧洲，慢性创伤就会转化为700万老年人的日常痛苦。今天使用的现代敷料不足以最佳地治疗这些伤口，因为大多数情况下，它们不包括积极促进伤口愈合的化合物。促进血管生成(伤口血管化)是慢性创面愈合的关键要求和合理的治疗方法。然而，包括蛋白质类血管刺激物的伤口敷料令人失望，因为这些刺激物高度不稳定，不能在治疗地点保持在治疗水平。在这里，我们建议测试促进伤口愈合的小而稳定的血管刺激和成纤维细胞刺激分子，在我们的实验室从一种中草药植物中分离出来。我们将利用平面测量法和高级组织化学方法，在动物和嵌合体皮肤模型上测试该刺激剂对伤口闭合的速度和完整性以及对再上皮化组织质量的影响。此外，我们还将通过核磁共振、质谱学、元素分析和结晶学等手段确定该分子的化学结构。综上所述，该项目旨在提供一种原理证明，即一种小型稳定的血管生成/成纤维细胞刺激器可以显著促进伤口愈合，并导致一种新的、商业上可行的治疗慢性伤口的方法。

项目成果

SBD.4 stimulates regenerative processes in vitro, and wound healing in genetically diabetic mice and in human skin/severe-combined immunodeficiency mouse chimera.

SBD.4 可刺激遗传性糖尿病小鼠和人类皮肤/严重联合免疫缺陷小鼠嵌合体的体外再生过程和伤口愈合。

• DOI: 10.1111/j.1743-6109.2006.00166.x
• 发表时间: 2006
• 期刊: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
• 作者: Zhao,Hui;Mortezaei,Reza;Wang,Yanrong;Sheng,Xinsheng;Aria,Fariba;Bojanowski,Krzysztof
• 通讯作者: Bojanowski,Krzysztof

Angelica sinensis isolate SBD.4: composition, gene expression profiling, mechanism of action and effect on wounds, in rats and humans.

当归分离物 SBD.4：成分、基因表达谱、作用机制以及对大鼠和人类伤口的影响。

• DOI: 10.1684/ejd.2011.1599
• 发表时间: 2012
• 期刊: European journal of dermatology : EJD
• 作者: Zhao,Hui;Deneau,Joel;Che,GinnyOL;Li,Shang;Vagnini,Frederic;Azadi,Parastoo;Sonon,Roberto;Ramjit,Ravi;Lee,SimonMY;Bojanowski,Krzysztof
• 通讯作者: Bojanowski,Krzysztof