Core--HUMAN TISSUE RESOURCES

核心--人体组织资源

• 批准号: 6990137
• 负责人: A J GANDOLFI
• 金额: $ 7.13万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-19 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990137
• 关键词: biomedical facility; cancer registry /resource; cell line; disease /disorder model; integrins; laboratory mouse; laminin; laser capture microdissection; medical outreach /case finding; metalloendopeptidases; metastasis; neoplasm /cancer invasiveness; organ culture; prostate neoplasms; tissue /cell culture; tissue resource /registry

The Human Tissue Core Facility has been established under the leadership of a basic scientist and a pathologist to ensure that prostate tissue procurement and ultilization of the tissue is optimized. Material will be obtained immediately after surgical removal and will be partitioned into fresh material for organ and cell culture. Additional normal and malignant portions will be snap frozen for use by the research projects. The Core will also provide laser capture microdissection (LCM) of both paraffin-embedded and frozen material for the research projects. All relative information will be transmitted to Core D for database entry. Components of Core B: 1. Tissue procurement 2. Establish organ cultures 3. Establish primary prostate cell lines 4. Laser capture microdissection (LCM) of normal and neoplastic glands.

人体组织核心设施是在基础科学家和病理学家的领导下建立的，以确保前列腺组织的获取和组织的利用得到优化。手术切除后立即获得材料，并将其分成新鲜材料用于器官和细胞培养。其他正常和恶性部分将被快速冷冻以供研究项目使用。该核心还将为研究项目提供石蜡包埋和冷冻材料的激光捕获显微切割（LCM）。所有相关信息将传输至Core D以进行数据库录入。核心B的组成部分： 1. 组织获取 2. 建立器官培养物 3. 建立原代前列腺细胞系 4. 正常腺体和肿瘤腺体的激光捕获显微切割 (LCM)。