PARACRINE REGULATION OF BILE DUCT SECRETION BY ATP

说明(改编自应用程序) 以下是临床科学家发展导师奖(K08)的申请表 要求为应聘者提供五年的指导职业生涯 走向学术独立的发展。应聘者是助理 耶鲁大学医学院的研究科学家，已经完成了两项以上的研究 多年的实验室培训，以及胃肠病学的临床培训。 候选人之前的工作重点是胆汁分泌的调节和 胞浆钙的控制。在拟议的研究项目中，候选人 将通过测试确定ATP在胆管分泌中的功能作用 具体目标如下：(1)确定哪些P2Y-ATP受体是 表达于胆管上皮细胞，(2)鉴定胆管上皮细胞膜分布 胆管上皮细胞上存在的P2Y-ATP受体，以及(3) 确定哪些P2YATP受体与胆管上皮细胞分泌有关。 候选人将利用这一奖项发展监管方面的科学专业知识 胆管分泌物，这将导致学术独立。这个 申请者的导师是第二信使中的既定调查员 调节上皮分泌，特别是与肝脏有关的分泌，以及 将在获奖期间大力支持候选人。这个 候选人将可以使用耶鲁最先进的设施，以及 有机会根据需要通过适当的课程来扩展自己的能力。 耶鲁大学医学院内科系承诺 候选人将拥有广泛的受保护的研究时间和优秀的 在授权期内提供的资源。

DESCRIPTION (adapted from the application) This application for a Mentored Clinical Scientist Development Award (K08) is requested to provide the candidate with five years of mentored career development leading to academic independence. The candidate is an Associate Research Scientist at Yale School of Medicine who has completed more than two years of laboratory training, as well as clinical training in Gastroenterology. The candidate's prior work has focused on regulation of bile secretion and control of cytosolic calcium. In the proposed research project, the candidate will define the functional role of ATP in bile ductular secretion by testing the following specific aims: (1) to identify which P2Y ATP receptors are expressed on bile duct epithelia, (2) to identify the membrane distribution of P2Y ATP receptors which are present on bile duct epithelia, and (3) to determine which P2Y ATP receptors link to bile duct epithelial secretion. The candidate will use this award to develop scientific expertise in regulation of bile ductular secretion, is which will lead to academic independence. The applicant's mentor is an established investigator in second messenger regulation of epithelial secretion, especially as it pertains to the liver, and will support the candidate strongly for the duration of the award. The candidate will have access to state-of-the-art facilities at Yale, as well as opportunities to expand his abilities through appropriate coursework as needed. The Yale School of Medicine Department of Internal Medicine has pledged that the candidate will have extensive protected research time and excellent resources for the duration of the award.