Development of a thermal cycling unit for DNA amplification using polymerase chain reaction in a microfluidic platform

在微流体平台中使用聚合酶链式反应开发用于 DNA 扩增的热循环装置

• 批准号: 460741-2013
• 负责人: Ren, Carolyn
• 金额: $ 1.82万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=542269
• 关键词: Development; thermal; cycling; unit; DNA

uFluidix is committed to developing novel Lab-on-a-Chip (LOC) instruments for a wide range of applications. One of uFluidix's current projects is to develop and commercialize a hand-held LOC instrument that utilizes microfluidic technology for on-farm detection of Porcine Reproductive and Respiratory Syndrome (PRRS) disease. PRRS remains an incurable infectious disease and causes significant economic and social stresses to the farm industry every year. One of the promising solutions is effective prevention through containment of the virus which requires a portable on-farm diagnostics tool to differentiate sick from healthy pigs. Polymerase chain reaction (PCR) which is a powerful technique for DNA amplification has proven to be successful for disease diagnosis. Therefore a LOC instrument integrated with microfluidic channels for realizing PCR

uFluidix致力于为广泛的应用开发新颖的芯片实验室（Lab-on-a-Chip，简称CUP）仪器。uFluidix目前的项目之一是开发和商业化一种手持式微流控仪器，该仪器利用微流控技术在农场检测猪繁殖与呼吸综合征（PRRS）疾病。PRRS仍然是一种无法治愈的传染病，每年都会给农业造成重大的经济和社会压力。其中一个有希望的解决方案是通过遏制病毒进行有效预防，这需要一种便携式农场诊断工具来区分患病猪和健康猪。聚合酶链反应（PCR）是一种强大的DNA扩增技术，已被证明是成功的疾病诊断。因此，一种集成了微流控通道的PCR检测仪