Core--Flow Cytometry

流式细胞仪共享设施自1985年以来一直存在，并为中心所有研究项目的许多癌症中心成员提供了基本的流式细胞仪服务。该中心的使命一直是服务于印第安纳大学癌症中心研究人员的各种研究需求。我们的目标是继续为癌症中心成员提供具有成本效益的最先进技术。使用这一设施所产生的大量出版物清单证明了其有效性。目前正在进行流式细胞术活动 由三名全职员工担任，他们都是受过广泛培训的合格操作员。该机构提供咨询、技术建议和协作。我们认为，提供这些服务的中央设施促进了尖端科学，也是癌症中心研究人员互动和交换科学信息的中心公共区域。自从这个设施成立以来，已经有许多人要求提供新的服务。随着新技术的加入，该设施提供的服务清单也在增加。该设施提供的人员和仪器几乎能够执行癌症中心调查人员要求的任何流式细胞仪分析。因此，可用的服务清单包括：1.多参数(4、5或6色)免疫荧光分析和细胞分选；2.DNA含量和细胞周期分析；3.同时免疫荧光和细胞周期分析；4.单细胞分选；5.细胞分选(散装或 7.BrdU掺入；8.活力分析；9.染色体分析(单变量和双变量)；10.受体-配体相互作用；11.动力学分析；以及12.离线数据分析。

The Flow Cytometry shared facility has been in existence since 1985 and has provided essential flow cytometric services to many Cancer Center members from all research programs of the Center. The mission of this facility has been to serve the varied research needs of researchers at the IU Cancer Center. Our goal is to continue to provide Cancer Center members access to cost-effective state-of-the-art technology. A substantial list of publications resulting from the use of this facility documents its effectiveness. Flow Cytometry activities are currently carried out by three full-time employees, all of whom are well qualified operators that have received extensive training. The facility provides consultation, technical advice, and collaboration. We believe that a centralized facility providing these services promotes cutting edge science and also serves as a central common area for Cancer Center investigators to interact and exchange scientific information. Since the inception of this facility, there have been many requests for new services. As new techniques were added, the list of services provided by the facility has grown. The personnel and instrumentation available at the facility are capable of performing virtually any flow cytometric assay requested by Cancer Center investigators. The list of available services thus includes: 1. Multiparameter (4, 5 or 6-color) immunofluorescence analysis and cell sorting; 2. DNA content and cell cycle analysis; 3. Simultaneous immunofluorescence and cell cycle analysis; 4. Single cell sorting; 5. Cell sorting (bulk or single cells) based on position of cells in cell cycle; 6. Apoptosis analysis (Annexin V/PI, Caspase, TUNEL and other assays); 7. BrdU incorporation; 8. Viability assays; 9. Chromosome analysis (univariate and bivariate); 10. Receptor - ligand interactions; 11. Kinetic analyses; and 12. Off-line data analysis.