Flow and Tissue Cytometry Core

摘要 定量流式细胞术和组织细胞术核心（FTCC，核心3）被提议为合作中心， 卓越的血液学（CCEH），以支持该中心的成员的研究工作。流式细胞术是 是绝大多数CCEH研究人员不可或缺的研究工具。在25名现任CCEH成员中， 在他们的研究中经常使用经典的流式细胞仪服务，超过65%的出版物 在过去的10年中，使用流式细胞仪服务。然而，许多人需要解释流式细胞术， 在造血生态位的空间关系的背景下的信息;例如，以确定如何 细胞相互作用，并与细胞和物理组成部分的生态位在正常和应激 条件为了满足这一需求，FTCC将通过独特的成像服务补充流式细胞术， 包括3D组织细胞术和活体显微镜。通过这种方式，核心将为CCEH成员提供 关键的服务沿着的机会，以扩大他们的审讯造血细胞，包括 定量分析HSC在不同条件下与其他细胞的空间分布， 非恶性造血FTCC利用现有的共享设施和专业知识， 印第安纳州大学医学院（IUSM）为CCEH成员提供最新的技术 和服务预期形成一个复杂的流式细胞术和组织细胞术设施。FTCC将提供一个成本- 为CCEH研究人员提供有效的资源，他们可以利用这一核心来应用强大的综合方法 非恶性血液学的研究，这将是一个宝贵的资产，他们的研究，以及 全国的血液学研究者FTCC的具体目标是：1-提供杰出的， 一致、及时和经济的流式细胞仪分析、细胞分选、图像流分析和单细胞 对所有CCEH成员进行蛋白质组学分析，以促进他们在造血和干细胞方面的研究 细胞生物学需要这些服务。2-提高CCEH成员的生产力， 中央化和标准化的流式细胞仪和其他相关服务， 印第安纳州大学内外的研究人员之间的协议和结果，并推进分发 科学知识。3-为CCEH成员提供大规模3D和高度复用的2D组织 细胞计数服务，从研究设计、荧光标记、图像收集、定量图像 分析和监督和无监督的数据分析。4-为CCEH成员提供活体显微镜检查 服务，包括机构合规性文件的准备、研究设计、动物手术、图像 收集和定量图像分析。这一安排将使CCEH研究人员和所有研究人员 全国范围内，强大的新工具，为他们的非恶性血液学研究。

ABSTRACT A quantitative Flow and Tissue Cytometry core (FTCC, core 3) is proposed for the Cooperative Center of Excellence in Hematology (CCEH) to support the research efforts of members of the center. Flow cytometry is an integral research tool for the vast majority of CCEH investigators. Of the 25 current CCEH members, 16 routinely utilize classical flow cytometric services in their research, and more than 65% of their publications over the last 10 years utilized flow cytometric services. However, many need to interpret flow cytometry information in the context of the spatial relationships in the hematopoietic niche; for example, to identify how cells interact with one another and with cellular and physical components of the niche in normal and stressed conditions. To address this need, the FTCC will complement flow cytometry with unique imaging services, including 3D tissue cytometry and intravital microscopy. In this way, the core will offer CCEH members a critical service along with the opportunity to extend their interrogation of hematopoietic cells to include quantitative analyses of the spatial distribution of HSC with other cells during different conditions of nonmalignant hematopoiesis. The FTCC capitalizes on existing shared facilities and expertise present within the Indiana University School of Medicine (IUSM) to provide CCEH members with the latest in technologies and services expected form a sophisticated flow and tissue cytometry facility. The FTCC will provide a cost- effective resource to CCEH investigators who can utilize this core to apply a powerful and integrated approach to studies of non-malignant hematology that will be an invaluable asset to their research, as well as to hematology investigators across the country. The specific aims of the FTCC are: 1- Provide outstanding, consistent, timely, and economical multicolor analysis, cell sorting, image flow analysis, and single cell proteomic analysis to all CCEH members to facilitate the conduct of their research in hematopoiesis and stem cell biology that requires any of these services. 2- Enhance the productivity of CCEH members by providing centralized and standardized flow cytometric and other related services that allow for the exchange of protocols and results between investigators within and outside Indiana University and advance the distribution of scientific knowledge. 3-Provide CCEH members with large-scale 3D and highly-multiplexed 2D tissue cytometry services, extending from study design, fluorescence labeling, image collection, quantitative image analysis and supervised and unsupervised data analysis. 4- Provide CCEH members with intravital microscopy services, including preparation of institutional compliance documents, study design, animal surgery, image collection and quantitative image analysis. This arrangement will give CCEH investigators and all investigators Nationwide, powerful new tools for their nonmalignant hematology investigations.