Core A- Cell Purification and Analysis Laboratory Core

核心A-细胞纯化和分析实验室核心

• 批准号: 6879599
• 负责人: Edward F Srour
• 金额: $ 17.11万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-29 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6879599
• 关键词: CD34 molecule; biomedical facility; cell population study; flow cytometry; hematopoietic stem cells; immunocytochemistry; tissue /cell culture; tissue /cell preparation

A shared cell procurement and analysis laboratory is proposed for the program project. This laboratory will serve an essential function in all four subprojects of the program project. This core will coordinate and perform flow cytometry and immunohistochemistry for purification and/or analysis of hematopoietic cells for the proposed studies. The present flow cytometry research laboratory which will be part of the proposed core unit is fully equipped with state of the art instrumentation for the performance of sophisticated flow cytometry procedures required by the proposed studies. The flow cytometry laboratory is currently located in a separate 400 square feet room on the fifth floor of the Medical Research and Library Building and will be relocated into a larger laboratory space on the first floor of the new Research Building projected to be completed in 1996. The laboratory activity is currently supported by two full time employees both of whom are well qualified operators who have received extensive training in flow cytometry. An additional employee is expected to join the core unit with the sole responsibility of procuring and preparing tissues and sorted cell populations for researchers involved in the proposed program project. The shared cell procurement and analysis laboratory will provide for participants in the program project an economical use of equipment and space as well as necessary cell populations required for the conduct of the research. The immunohistology laboratory which will be part of the proposed core unit is fully equipped to immunocharacterize animal and human hematopoietic and non-hematopoietic cells using antibodies specific for cell derivation, cell activation or oncogene products. In addition the laboratory can perform cytokinetic analysis of the specimens using the monoclonal antibodies and can assess cell apoptosis by a peroxidase DNA and labeling technique. The laboratory is located in a 210 square feet facility within the Division of Hematopathology of the Department of Pathology at the fourth floor of the I.U. Hospital. The laboratory is currently staffed by two full time techologists who have extensive experience in both research and routine immunohistology.

提议建立一个共用的细胞采购和分析实验室 计划项目。这个实验室将在世界各地发挥重要作用 该计划项目的四个子项目。这个核心将协调和 进行流式细胞术和免疫组织化学纯化和/或 对建议研究的造血细胞进行分析。现在 流式细胞仪研究实验室将成为拟议的 核心单元完全配备了最先进的仪器设备， 执行复杂的流式细胞术程序所需的 建议进行的研究。流式细胞仪实验室目前位于 医疗中心五楼一间400平方英尺的独立房间 研究和图书馆大楼，将搬迁到一个更大的 新研究大楼一楼的实验室空间 预计将于1996年完工。实验室活动目前正在进行 由两名全职员工支持，这两名员工都很有资格 接受过广泛的流式细胞仪培训的操作员。一个 预计将有更多的员工加入核心单位 获取和准备组织和分选细胞的责任 为参与拟议计划项目的研究人员提供人口。 共享细胞采购和分析实验室将提供 该计划的参与者计划节约使用设备和 所需的空间和必要的细胞数量 这项研究。 免疫组织学实验室，这将是拟议核心的一部分 设备齐全，可对动物和人类进行免疫学鉴定 使用特异性抗体的造血细胞和非造血细胞 细胞衍生、细胞激活或癌基因产物。此外， 实验室可以使用 单抗，可通过过氧化物酶DNA评估细胞凋亡 和标记技术。该实验室占地210平方英尺。 年系血液病理科内的设施 在I.U医院四楼的病理科。实验室是 目前由两名全职技术专家组成，他们拥有广泛的 有研究和常规免疫组织学方面的经验。