BD FACSAria System

BD FACSAria 系统

• 批准号: 8447164
• 负责人: Edward F Srour
• 金额: $ 51.57万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-20 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447164
• 关键词: 13 year old; Agreement; Calendar; Cancer Center; Cell Separation; Cells; Containment; Cytometry; Environment; Extramural Activities; Fee-for-Service Plans; Flow Cytometry; Funding; Grant; Guidelines; Health Services Accessibility; Hour; Indiana; International; Investigation; Laboratories; Lasers; Policies; Productivity; Research; Research Personnel; Resources; Sampling; Science; Secure; Services; Societies; System; Technology; United States National Institutes of Health; Universities; base; cell type; institutional biosafety committee; instrument; medical schools

DESCRIPTION (provided by applicant): The Flow Cytometry Resource Facility (FCRF) at the Indiana University Simon Cancer Center (IUSCC) is a centralized shared facility providing multiple flow cytometric services to over 100 investigators at the Indiana University School of Medicine (IUSM). The FCRF is a strictly research-dedicated, fee-for-service core laboratory with flow cytometric cell sorting being a major service provided by this facility. The FCRF was established in 1985 and has been part of the Indiana University Simon Cancer Center since 1992. During Calendar-Year 2010, forty-four investigators used over 1600 hours of cell sorting and in the last 7 years, the number of investigators using cell sorting services at the FCRF ranged between 41 and 53 per year. The majority of these investigators hold federally funded grants (primarily R01, P01 and R21 NIH grants and similar DoD grants). Many of the cell samples subjected to cell sorting by different users are classified as BSL2 or BSL3 and the handling of these cells should therefore follow guidelines established by the Institutional Biosafety Committee, the International Society of Analytical Cytometry Biosafety Guidelines, and the developing NIH-wide standards for biosafety in cell sorting laboratories. At present, one of the 3 sorters in the FCRF is a 13-year old FACSVantage SE that cannot be maintained under a service agreement anymore and that cannot be reconfigured to comply with any of the new biocontainment requirements for the safe handling of biohazardous materials. We are therefore requesting funds to acquire a Special Becton Dickinson FACSAria System for cell sorting composed of five lasers and contained completely within a BSL2/BSL3 biosafety cabinet. Our justification of need for this instrument is based on three critical necessities. First, BD does no service our FACSVantage SE anymore. In fact, since the original submission of this application, this instrument has been technically out of commission. Second, the evolving policies on biosafety levels, containment, and practices in flow cytometry by many governing bodies require the use of cell sorters with BSL2/BSL3 containment systems with many of the cell types subjected to cell sorting at the FCRF and which cannot be accomplished by the FACSVantage SE. Third, technological advancements in flow cytometry in general and cell sorting in particular make the FACSVantage SE a rather ineffective and inefficient cell sorter for most users wishing to apply state-of-the-art technologies in their research. These hard realities are at present creating a considerable strain on the availability of services provided by the FCRF for investigators and are beginning in some cases to hinder their productivity and competitiveness in securing extramural funding for their research. Acquisition of this new cell sorter should allow almost fifty investigators at the IUSM to conduct their research for years to come within a safe and compliant biocontainment environment and to benefit from state-of-the-art technologies that promote first class investigations and enhance cutting edge science.

描述（由申请人提供）：印第安纳大学西蒙癌症中心 (IUSCC) 的流式细胞术资源设施 (FCRF) 是一个集中共享设施，为印第安纳大学医学院 (IUSM) 的 100 多名研究人员提供多种流式细胞术服务。 FCRF 是一个严格致力于研究、按服务收费的核心实验室，流式细胞术细胞分选是该设施提供的主要服务。 FCRF 成立于 1985 年，自 1992 年起成为印第安纳大学西蒙癌症中心的一部分。2010 日历年期间，44 名研究人员使用了超过 1600 小时的细胞分选服务，在过去 7 年中，每年使用 FCRF 细胞分选服务的研究人员数量在 41 至 53 名之间。这些研究人员中的大多数拥有联邦资助的拨款（主要是 R01、P01 和 R21 NIH 拨款以及类似的 DoD 拨款）。许多由不同用户进行细胞分选的细胞样本被归类为 BSL2 或 BSL3，因此这些细胞的处理应遵循机构生物安全委员会、国际分析细胞计量学会生物安全指南以及正在制定的 NIH 细胞分选实验室生物安全标准制定的指南。目前，FCRF 中的 3 台分拣机之一是已有 13 年历史的 FACSVantage SE，无法再根据服务协议进行维护，也无法重新配置以符合安全处理生物危险材料的任何新生物防护要求。因此，我们请求资金购买用于细胞分选的特殊 Becton Dickinson FACSAria 系统，该系统由五个激光器组成，并完全包含在 BSL2/BSL3 生物安全柜内。我们需要该工具的理由是基于三个关键的必要条件。首先，BD 不再为我们的 FACSVantage SE 提供服务。事实上，自从最初提交该申请以来，该仪器在技术上就已经无法使用。其次，许多管理机构不断发展的关于生物安全水平、遏制和流式细胞术实践的政策要求使用带有 BSL2/BSL3 遏制系统的细胞分选仪，其中许多细胞类型在 FCRF 上进行细胞分选，而 FACSVantage SE 无法完成这一任务。第三，流式细胞术的技术进步，特别是细胞分选的技术进步，使得 FACSVantage SE 对于大多数希望在研究中应用最先进技术的用户来说是一种相当低效和低效的细胞分选仪。这些严峻的现实目前对 FCRF 为研究人员提供的服务造成了相当大的压力，并且在某些情况下开始阻碍他们在为研究获取外部资金方面的生产力和竞争力。购买这种新的细胞分选仪应该可以 IUSM 的近 50 名研究人员将在安全、合规的生物防护环境中进行多年的研究，并受益于促进一流研究和增强尖端科学的最先进技术。