BD FACSAria System

描述（由申请人提供）：印第安纳州西蒙大学癌症中心（IUSCC）的流式细胞术资源设施（FCRF）是一个集中共享设施，为印第安纳州大学医学院（IUSM）的100多名研究者提供多种流式细胞术服务。FCRF是一个严格的研究专用，收费服务的核心实验室，流式细胞仪细胞分选是该设施提供的主要服务。FCRF成立于1985年，自1992年以来一直是印第安纳州西蒙大学癌症中心的一部分。在2010日历年度，44名研究者使用了超过1600小时的细胞分选，在过去的7年中，在FCRF使用细胞分选服务的研究者人数在每年41至53人之间。这些研究人员中的大多数持有联邦资助的赠款（主要是R 01，P01和R21 NIH赠款和类似的国防部赠款）。由不同用户进行细胞分选的许多细胞样本被归类为BSL 2或BSL 3，因此这些细胞的处理应遵循机构生物安全委员会制定的指南、国际分析细胞计数学会生物安全指南以及正在制定的NIH范围内细胞分选实验室生物安全标准。目前，FCRF中的3台分拣机中有一台是使用了13年的FACSVantage SE，无法再根据服务协议进行维护，也无法重新配置以符合任何新的生物防护要求，从而安全处理生物危害材料。因此，我们正在申请资金，以获得一个特殊的Becton Dickinson FACSAria系统，用于细胞分选，该系统由五个激光器组成，完全包含在BSL 2/BSL 3生物安全柜中。我们认为需要这一文书的理由是基于三个关键的必要性。首先，BD不再为我们的FACSVantage SE提供服务。事实上，自从最初提交本申请以来，这一文书在技术上已经失效。其次，许多管理机构不断发展的生物安全水平、防护和流式细胞术实践政策要求使用带有BSL 2/BSL 3防护系统的细胞分选仪，其中许多细胞类型在FCRF进行细胞分选，而FACSVantage SE无法完成。第三，流式细胞术的技术进步，特别是细胞分选，使得FACSVantage SE对于大多数希望在研究中应用最先进技术的用户来说，是一种相当无效和低效的细胞分选仪。目前，这些严峻的现实对联邦研究基金为研究人员提供的服务造成了相当大的压力，在某些情况下，开始阻碍他们为研究争取校外资金的生产力和竞争力。购买这种新的细胞分选仪应该可以让 近50名研究人员在IUSM进行他们的研究多年来在一个安全和合规的生物遏制环境，并受益于国家的最先进的技术，促进一流的调查和提高尖端科学。

DESCRIPTION (provided by applicant): The Flow Cytometry Resource Facility (FCRF) at the Indiana University Simon Cancer Center (IUSCC) is a centralized shared facility providing multiple flow cytometric services to over 100 investigators at the Indiana University School of Medicine (IUSM). The FCRF is a strictly research-dedicated, fee-for-service core laboratory with flow cytometric cell sorting being a major service provided by this facility. The FCRF was established in 1985 and has been part of the Indiana University Simon Cancer Center since 1992. During Calendar-Year 2010, forty-four investigators used over 1600 hours of cell sorting and in the last 7 years, the number of investigators using cell sorting services at the FCRF ranged between 41 and 53 per year. The majority of these investigators hold federally funded grants (primarily R01, P01 and R21 NIH grants and similar DoD grants). Many of the cell samples subjected to cell sorting by different users are classified as BSL2 or BSL3 and the handling of these cells should therefore follow guidelines established by the Institutional Biosafety Committee, the International Society of Analytical Cytometry Biosafety Guidelines, and the developing NIH-wide standards for biosafety in cell sorting laboratories. At present, one of the 3 sorters in the FCRF is a 13-year old FACSVantage SE that cannot be maintained under a service agreement anymore and that cannot be reconfigured to comply with any of the new biocontainment requirements for the safe handling of biohazardous materials. We are therefore requesting funds to acquire a Special Becton Dickinson FACSAria System for cell sorting composed of five lasers and contained completely within a BSL2/BSL3 biosafety cabinet. Our justification of need for this instrument is based on three critical necessities. First, BD does no service our FACSVantage SE anymore. In fact, since the original submission of this application, this instrument has been technically out of commission. Second, the evolving policies on biosafety levels, containment, and practices in flow cytometry by many governing bodies require the use of cell sorters with BSL2/BSL3 containment systems with many of the cell types subjected to cell sorting at the FCRF and which cannot be accomplished by the FACSVantage SE. Third, technological advancements in flow cytometry in general and cell sorting in particular make the FACSVantage SE a rather ineffective and inefficient cell sorter for most users wishing to apply state-of-the-art technologies in their research. These hard realities are at present creating a considerable strain on the availability of services provided by the FCRF for investigators and are beginning in some cases to hinder their productivity and competitiveness in securing extramural funding for their research. Acquisition of this new cell sorter should allow almost fifty investigators at the IUSM to conduct their research for years to come within a safe and compliant biocontainment environment and to benefit from state-of-the-art technologies that promote first class investigations and enhance cutting edge science.