BD FACSAria System

描述（由申请人提供）：印第安纳大学西蒙癌症中心（IUSCC）的流式细胞术资源设施（FCRF）是一个集中式共享设施，为印第安纳大学医学院（usm）的100多名研究人员提供多种流式细胞术服务。FCRF是一个严格的研究专用，收费服务的核心实验室，流式细胞术细胞分选是该设施提供的主要服务。FCRF成立于1985年，自1992年以来一直是印第安纳大学西蒙癌症中心的一部分。在2010日历年期间，44名调查人员使用了1600多个小时的细胞分类，在过去7年中，在FCRF使用细胞分类服务的调查人员数量在每年41至53人之间。这些研究人员中的大多数持有联邦资助的补助金（主要是R01， P01和R21 NIH补助金以及类似的国防部补助金）。许多由不同用户进行细胞分选的细胞样本被分类为BSL2或BSL3，因此，这些细胞的处理应遵循机构生物安全委员会、国际分析细胞术学会生物安全指南以及正在制定的美国国立卫生研究院范围内的细胞分选实验室生物安全标准所制定的指南。目前，FCRF的3台分选机中有一台使用了13年的facadvantage SE分选机已经无法再根据服务协议进行维护，也无法重新配置以符合安全处理生物有害材料的任何新的生物防护要求。因此，我们请求资金购买一个特殊的Becton Dickinson FACSAria系统，用于细胞分选，该系统由五个激光器组成，完全包含在BSL2/BSL3生物安全柜中。我们认为需要这一文书的理由是基于三个关键的需要。首先，BD不再为我们的facadvantage SE提供服务。事实上，自最初提交此申请以来，该文书在技术上已经停止使用。其次，许多管理机构在生物安全水平、控制和流式细胞术实践方面不断发展的政策要求使用具有BSL2/BSL3控制系统的细胞分选器，其中许多细胞类型需要在FCRF进行细胞分选，而FACSVantage SE无法完成这一任务。第三，流式细胞术的技术进步，特别是细胞分选，使得facadvantage SE对于大多数希望在研究中应用最先进技术的用户来说，是一种相当低效的细胞分选器。这些严峻的现实目前对研究经费基金向研究人员提供的服务造成相当大的压力，并在某些情况下开始妨碍他们在为其研究争取外部资金方面的生产力和竞争力。获得这种新的细胞分选器应该允许

DESCRIPTION (provided by applicant): The Flow Cytometry Resource Facility (FCRF) at the Indiana University Simon Cancer Center (IUSCC) is a centralized shared facility providing multiple flow cytometric services to over 100 investigators at the Indiana University School of Medicine (IUSM). The FCRF is a strictly research-dedicated, fee-for-service core laboratory with flow cytometric cell sorting being a major service provided by this facility. The FCRF was established in 1985 and has been part of the Indiana University Simon Cancer Center since 1992. During Calendar-Year 2010, forty-four investigators used over 1600 hours of cell sorting and in the last 7 years, the number of investigators using cell sorting services at the FCRF ranged between 41 and 53 per year. The majority of these investigators hold federally funded grants (primarily R01, P01 and R21 NIH grants and similar DoD grants). Many of the cell samples subjected to cell sorting by different users are classified as BSL2 or BSL3 and the handling of these cells should therefore follow guidelines established by the Institutional Biosafety Committee, the International Society of Analytical Cytometry Biosafety Guidelines, and the developing NIH-wide standards for biosafety in cell sorting laboratories. At present, one of the 3 sorters in the FCRF is a 13-year old FACSVantage SE that cannot be maintained under a service agreement anymore and that cannot be reconfigured to comply with any of the new biocontainment requirements for the safe handling of biohazardous materials. We are therefore requesting funds to acquire a Special Becton Dickinson FACSAria System for cell sorting composed of five lasers and contained completely within a BSL2/BSL3 biosafety cabinet. Our justification of need for this instrument is based on three critical necessities. First, BD does no service our FACSVantage SE anymore. In fact, since the original submission of this application, this instrument has been technically out of commission. Second, the evolving policies on biosafety levels, containment, and practices in flow cytometry by many governing bodies require the use of cell sorters with BSL2/BSL3 containment systems with many of the cell types subjected to cell sorting at the FCRF and which cannot be accomplished by the FACSVantage SE. Third, technological advancements in flow cytometry in general and cell sorting in particular make the FACSVantage SE a rather ineffective and inefficient cell sorter for most users wishing to apply state-of-the-art technologies in their research. These hard realities are at present creating a considerable strain on the availability of services provided by the FCRF for investigators and are beginning in some cases to hinder their productivity and competitiveness in securing extramural funding for their research. Acquisition of this new cell sorter should allow almost fifty investigators at the IUSM to conduct their research for years to come within a safe and compliant biocontainment environment and to benefit from state-of-the-art technologies that promote first class investigations and enhance cutting edge science.