BD FACSAria System

描述(由申请人提供)：印第安纳大学西蒙癌症中心(IUSCC)的流式细胞术资源设施(FCRF)是一个集中式共享设施，为印第安纳大学医学院(IUSM)的100多名研究人员提供多项流式细胞仪服务。FCRF是一个严格致力于研究、收费的核心实验室，流式细胞仪细胞分选是该设施提供的一项主要服务。FCRF成立于1985年，自1992年以来一直是印第安纳大学西蒙癌症中心的一部分。在2010历年，44名调查人员使用了超过1600个小时的细胞分类，在过去7年中，FCRF每年使用细胞分类服务的调查人员数量在41到53人之间。这些调查人员中的大多数拥有联邦资助的补助金(主要是R01、P01和R21 NIH补助金和类似的国防部补助金)。许多由不同用户进行细胞分选的细胞样本被归类为BSL2或BSL3，因此，这些细胞的处理应遵循机构生物安全委员会、国际分析细胞测量生物安全指南以及正在制定的NIH范围内细胞分选实验室生物安全标准。目前，FCRF中的3个分拣机中有一个是已有13年历史的FACSVantage SE，它不能再根据服务协议进行维护，也不能重新配置以符合安全处理生物危险材料的任何新的生物遏制要求。因此，我们正在申请资金，以购买一种特殊的Becton Dickinson FACSAria系统，用于细胞分选，该系统由五个激光器组成，完全包含在BSL2/BSL3生物安全柜中。我们需要这一工具的理由是基于三个关键的必要性。首先，BD不再为我们的FACSVantage SE提供服务。事实上，自本申请书最初提交以来，该文书在技术上已经失效。其次，许多理事机构关于生物安全水平、遏制和流式细胞术实践的不断演变的政策要求将细胞分选机与BSL2/BSL3遏制系统一起使用，其中许多细胞类型在FCRF接受细胞分离，这不是FACSVantage SE所能完成的。第三，FACSVantage SE在流式细胞术特别是细胞分选方面的技术进步使其对于希望在研究中应用最新技术的大多数用户来说是一个相当低效和低效的细胞分选机。这些严峻的现实目前正在对森林资源保护框架为调查人员提供的服务造成相当大的压力，并在某些情况下开始阻碍他们在为其研究争取外部资金方面的生产力和竞争力。购买这种新的细胞分选器应该可以 近50名研究人员在IUSM进行了多年的研究，以便在安全和合规的生物遏制环境中进行研究，并受益于促进一流调查和加强尖端科学的最先进技术。

DESCRIPTION (provided by applicant): The Flow Cytometry Resource Facility (FCRF) at the Indiana University Simon Cancer Center (IUSCC) is a centralized shared facility providing multiple flow cytometric services to over 100 investigators at the Indiana University School of Medicine (IUSM). The FCRF is a strictly research-dedicated, fee-for-service core laboratory with flow cytometric cell sorting being a major service provided by this facility. The FCRF was established in 1985 and has been part of the Indiana University Simon Cancer Center since 1992. During Calendar-Year 2010, forty-four investigators used over 1600 hours of cell sorting and in the last 7 years, the number of investigators using cell sorting services at the FCRF ranged between 41 and 53 per year. The majority of these investigators hold federally funded grants (primarily R01, P01 and R21 NIH grants and similar DoD grants). Many of the cell samples subjected to cell sorting by different users are classified as BSL2 or BSL3 and the handling of these cells should therefore follow guidelines established by the Institutional Biosafety Committee, the International Society of Analytical Cytometry Biosafety Guidelines, and the developing NIH-wide standards for biosafety in cell sorting laboratories. At present, one of the 3 sorters in the FCRF is a 13-year old FACSVantage SE that cannot be maintained under a service agreement anymore and that cannot be reconfigured to comply with any of the new biocontainment requirements for the safe handling of biohazardous materials. We are therefore requesting funds to acquire a Special Becton Dickinson FACSAria System for cell sorting composed of five lasers and contained completely within a BSL2/BSL3 biosafety cabinet. Our justification of need for this instrument is based on three critical necessities. First, BD does no service our FACSVantage SE anymore. In fact, since the original submission of this application, this instrument has been technically out of commission. Second, the evolving policies on biosafety levels, containment, and practices in flow cytometry by many governing bodies require the use of cell sorters with BSL2/BSL3 containment systems with many of the cell types subjected to cell sorting at the FCRF and which cannot be accomplished by the FACSVantage SE. Third, technological advancements in flow cytometry in general and cell sorting in particular make the FACSVantage SE a rather ineffective and inefficient cell sorter for most users wishing to apply state-of-the-art technologies in their research. These hard realities are at present creating a considerable strain on the availability of services provided by the FCRF for investigators and are beginning in some cases to hinder their productivity and competitiveness in securing extramural funding for their research. Acquisition of this new cell sorter should allow almost fifty investigators at the IUSM to conduct their research for years to come within a safe and compliant biocontainment environment and to benefit from state-of-the-art technologies that promote first class investigations and enhance cutting edge science.