Pain Modulation and Visceral Stimulation

疼痛调节和内脏刺激

• 批准号: 6751909
• 负责人: Peggy Mason
• 金额: $ 28.5万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6751909
• 关键词: analgesia; cardiovascular function; electrocardiography; electromyography; gastrointestinal sign /symptom; heat stimulus; laboratory rat; lidocaine; lumbosacral region; microelectrodes; muscimol; neural conduction; neural information processing; neuropharmacology; pons; serotonin; visceral afferent nerve

DESCRIPTION (provided by applicant): Visceral nociception, like cutaneous nociception, is subject to descending modulatory influences from the medullary raphe magnus (RM). However, little is known about if and how RM cells contribute to: 1) visceral stimulus evoked nocifensive reactions; and 2) visceral stimulus evoked suppression of cutaneous nociception. The proposed experiments use colorectal distension (CRD) as a model visceral stimulus to explore these issues. There are 6 aims: . Aim 1A: Identify the spinal trajectory of afferents that carry ascending CRD information to RM cells. . Aim 1B: Identify the contributions of descending modulatory input, arising from RM and elsewhere, to CRD-evoked cardiovascular and visceromotor reactions. . Aim 2: Determine the effect of RM cellular inactivation on behavioral reactions to CRD. . Aim 3: Identify the physiological characteristics of neurons that discharge in a pro-nociceptive manner, with increasing excitatory responses to increasing intensities of CRD stimulation. . Aim 4: Determine the spinal pathway taken by descending modulatory input, from RM and elsewhere, to the lumbosacral spinal cord. . Aim 5: Establish the role of RM cellular activation in heterotopic suppression of cutaneous nociception by a noxious visceral stimulus. . Aim 6: Aim 3: Identify the physiological characteristics of neurons that may subserve the antinociceptive effects of CRD stimulation. The proposed experiments will test the novel hypothesis that RM's effects on spinal nociception consists of a "pro-nociceptive" component that is necessary for the normal behavioral reaction to a noxious visceral stimulus in addition to the better-studied "inhibitory modulation" component.

描述（由申请人提供）：内脏伤害性感受与皮肤伤害性感受一样，受到来自延髓中缝大肌（RM）的下行调节影响。然而，很少有人知道，如果和如何RM细胞有助于：1）内脏刺激诱发的伤害性反应;和2）内脏刺激诱发的皮肤伤害性感受的抑制。拟议的实验使用结直肠扩张（CRD）作为模型内脏刺激，以探讨这些问题。 有六个目标： . 目的1A：识别携带上行CRD信息到RM的传入神经的脊髓轨迹 细胞 . 目的1B：确定下行调制输入的贡献，来自RM和 在其他地方，CRD诱发的心血管和内脏反应。 . 目的2：确定RM细胞失活对CRD行为反应的影响。 . 目的3：识别在促伤害性感受中放电的神经元的生理特征。 方式，随着CRD刺激强度的增加，兴奋性反应增加。 . 目的4：确定下行调制输入从RM和 在其他地方，腰骶脊髓。 . 目的5：确定RM细胞活化在皮肤异位抑制中的作用。 由有害的内脏刺激引起的伤害感受。 . 目的6：目的3：确定可能有助于神经元的生理特征。 CRD刺激的抗伤害感受作用。 拟议的实验将测试新的假设，即RM对脊髓伤害性感受的影响包括一个“亲伤害性”的组成部分，这是必要的正常行为反应，除了更好地研究“抑制性调制”的组成部分的伤害性内脏刺激。