In Vivo Rabbit Model of Finger Muscoloskeletal Disorders

描述（由申请人提供）：与职业性肌肉骨骼疾病（MSD）相关的成本和发病率持续上升，需要对已知的损伤风险因素进行调查。重复运动、用力负荷及其组合（重复负荷）是MSD的风险因素。这些恰好是手和其他关节的MSD的少数可改变的风险因素之一。本项目拟使用重复性指关节负荷的体内动物模型来阐明这些因素（重复和合力和重复）的相对风险。手指关节负荷被用来模拟在工作场所发现的手密集型任务，以测试以下假设：力和重复的组合是一个职业风险因素的MSD。该项目将量化由于重复载荷引起的关节组织的结构和分子变化。最后，本计画将在剂量反应研究中确定重复负荷的临界值。本研究的长期目标是：1）确定手指关节负荷的个体生物力学特征的相对贡献，即重复、力水平和暴露持续时间; 2）证明这些生物力学风险因素、细胞反应、组织损伤和损伤之间的因果关系。该机械生物学项目的结果将为有效干预手关节的MSD（如骨关节炎（OA）或退行性关节病（DJD））提供指导。该项目直接解决了国家职业研究议程（诺拉）在1）开发一个定量的剂量-反应模型，确定剂量-反应关系，2）确定是否有更多的组织损伤反应与重复的负荷或峰值负荷水平，和3）确定超微结构损伤和生化改变与物理负荷。

DESCRIPTION (provided by applicant): The ongoing rise in costs and morbidity associated with occupational musculoskeletal disorders (MSDs) demands the investigation of known risk factors for injury. Repetition of movement, forceful loading and their combination (repetitive loading) are risk factors for MSDs. These happen to be some of the few modifiable risk factors for MSDs of the hand and other joints. This project proposes to use an in vivo animal model of repetitive finger joint loading to elucidate the relative risks of these factors (repetition and combined force and repetition). Finger joint loading is used to simulate hand intensive tasks found in the workplace to test the following hypothesis: The combination of force and repetition is an occupational risk factor for MSD. This project will quantify the structural and molecular changes in joint tissues due to repetitive loading. And finally, this project will determine the threshold of repetitive loading in a dose-response study. The long term goals of this study are to 1) determine the relative contributions of individual biomechanical characteristics of finger joint loading, namely repetition, force level, and duration of exposure; and 2) demonstrate causality between these biomechanical risk factors, cellular response, tissue damage, and injury. The results of this mechanobiology project will lead to guidelines for effective interventions of MSDs of the hand joints such as osteoarthritis (OA) or degenerative joint disease (DJD). This project directly addresses the National Occupational Research Agenda (NORA) in 1) developing a quantitative dose-response model identifying dose-response relationships, 2) determining whether the injury response of the tissue has more to do with repetition of loading or level of peak load, and 3) identifying the ultrastructural injury and biochemical alterations associated with physical loading.