Atomic Force Microscopy Instrumentation and Applications

原子力显微镜仪器和应用

• 批准号: 6837020
• 负责人: Paul D Smith
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6837020
• 关键词: atomic force microscopy; bioimaging /biomedical imaging; biomedical equipment development; clathrin; image enhancement; intermolecular interaction; intracellular membranes; intracellular transport; molecular dynamics; nanotechnology; protein biosynthesis; protein transport; receptor mediated endocytosis; temperature; vesicle /vacuole

The atomic force microscope (AFM) is becoming increasingly useful for studying ultra-structure and functional properties of biological molecules and tissue. The DBEPS Instrumentation Research and Development Resource is strengthening the AFM capabilities at NIH to support the diverse needs of IC scientific projects. An AFM instrument system has been obtained and is being adapted to allow measurements to be made of biological samples. A perfusion chamber has been developed to enable a single cell to be retained for examination and permitting manipulation of the extra-cellular environment. A temperature controlled environmental chamber has also been developed to allow samples that exhibit temperature dependent properties to be examined. The chamber is capable of 0.1 degree stability within a temperature range from 10 to 40 Celsius. Using DBEPS expertise in optics, electronics, mechanical design, and other areas, associated instrumentation and quantitative analysis methods are being pursued to advance AFM technology and apply it to solve novel biomedical problems. Collaborative intramural biological projects include the investigation of the viscoelastic energetics of the protein clathrin and its assemblies that are important to subcellular protein trafficking (NICHD) and surface modified protein interaction dynamics (NIDDK). Integration of AFM with near-field scanning optical microscopy (NIAID) is being pursued through a collaborative agreement with NIST to develop and adapt existing instrumentation for application to biological problems. Preliminary implementation of our novel methodology has been successful when applied to standard samples for calibration and validation. New AFM components are being acquired and integrated to expand our facility capabilities towards meeting the varying needs of intramural researchers from across NIH. This technology is being applied to studying the mechanical properties associated with vesicle formation and vesicular trafficking. In this particular project we examine the assembly of clathrin triskelions into polyhedral coats of about 100-nanometer diameter which is believed to play a central role in receptor-mediated endocytosis and intracellular trafficking from the trans-Golgi network. Knowledge of the mechanical properties of the clathrin coat is needed in order to fully understand the function of the coat in the dynamical control of vesicle formation. The objective here is to measure the mechanical properties of clathrin-coated and uncoated vesicles in biological fluid environments. We have developed a scheme by which the nanomechanics of both vesicle types can be quantitatively explored by AFM, employing a deformation force in the 50-100 pN range. The measurements are being refined via better instrument parameter calibrations and vesicle sample preparations.

原子力显微镜（AFM）在研究生物分子和组织的超微结构和功能特性方面正变得越来越有用。DBEPS仪器研究和开发资源正在加强NIH的AFM能力，以支持IC科学项目的各种需求。AFM仪器系统已经获得，并正在调整，以允许进行测量的生物样品。已经开发了灌注室，以使单个细胞能够被保留用于检查并允许操纵细胞外环境。还开发了温度控制环境室，以允许对表现出温度依赖性的样品进行检查。该室能够在10至40摄氏度的温度范围内保持0.1度的稳定性。利用DBEPS在光学、电子、机械设计和其他领域的专业知识，正在寻求相关的仪器和定量分析方法来推进AFM技术，并将其应用于解决新的生物医学问题。壁内生物学合作项目包括研究蛋白质网格蛋白及其组装体的粘弹性能，这对亚细胞蛋白质运输（NICHD）和表面修饰蛋白质相互作用动力学（NIDDK）非常重要。AFM与近场扫描光学显微镜（NIAID）的集成正在通过与NIST的合作协议进行，以开发和调整现有的仪器应用于生物问题。我们的新方法的初步实施已成功应用于标准样品的校准和验证。新的原子力显微镜组件正在收购和整合，以扩大我们的设施能力，以满足来自全国卫生研究院的内部研究人员的不同需求。 该技术正被应用于研究与囊泡形成和囊泡运输相关的机械性能。在这个特定的项目中，我们研究组装成多面体外套的网格蛋白triskelions约100纳米的直径，这被认为是发挥了核心作用，受体介导的内吞作用和细胞内运输的trans-Golgi网络。为了充分理解囊泡形成的动态控制中的外套的功能，需要网格蛋白外套的机械性能的知识。本文的目的是测量网格蛋白包被和未包被囊泡在生物流体环境中的机械性能。我们已经开发出一种方案，通过该方案，两种囊泡类型的纳米力学可以通过AFM进行定量探索，采用在50-100 pN范围内的变形力。通过更好的仪器参数校准和囊泡样品制备，测量结果正在得到改进。