Instrumentation for Spectral Karyotyping

光谱核型是一种进化中的细胞遗传学 识别染色体重排的技术，如 癌细胞系中可见的缺失、倒置和易位。一个 商业票据作为改进的Sagnac运行 安装在外照显微镜上的干涉仪 捕获用于绘制的探头的光谱特征 染色体。外延照明端口和荧光立方体 显微镜的装配已被改装成可单独使用 波长激发，导致大于一个数量级 在信噪比上的幅度提高 荧光信号和总背景信号的减少。 这些改进将允许更小的染色体的分辨 条带异常及其与疾病相关性的识别 疾病。

Spectral karyotyping is an evolving cytogenetic technology to identify chromosomal rearrangements such as deletions, inversions, and translocations seen in cancer cell lines. A commercial instrument operates as a modified Sagnac interferometer installed on an epi-illumination microscope to capture the spectral signature of probes used to paint chromosomes. The epi-illumination port and the fluorescent cube assembly of the microscope have been modified to permit single wavelength excitation, resulting in greater than an order of magnitude improvement in the signal to noise ratio of the fluorescent signals and a reduction in the overall background signal. These improvements will permit resolution of smaller chromosome band abnormalities and identification of their correlation with disease.