Instrumentation for Spectral Karyotyping

光谱核型分析是一种不断发展的细胞遗传学 识别染色体重排的技术，例如 癌细胞系中观察到的缺失、倒位和易位。一个 商业乐器作为改进的 Sagnac 运行 安装在落射照明显微镜上的干涉仪 捕获用于绘画的探针的光谱特征 染色体。落射照明端口和荧光立方体 显微镜的组件已被修改，以允许单个 波长激发，导致大于一个数量级 信噪比的幅度改善 荧光信号和总体背景信号的减少。 这些改进将允许分辨率更小的染色体 条带异常及其相关性的识别 疾病。

Spectral karyotyping is an evolving cytogenetic technology to identify chromosomal rearrangements such as deletions, inversions, and translocations seen in cancer cell lines. A commercial instrument operates as a modified Sagnac interferometer installed on an epi-illumination microscope to capture the spectral signature of probes used to paint chromosomes. The epi-illumination port and the fluorescent cube assembly of the microscope have been modified to permit single wavelength excitation, resulting in greater than an order of magnitude improvement in the signal to noise ratio of the fluorescent signals and a reduction in the overall background signal. These improvements will permit resolution of smaller chromosome band abnormalities and identification of their correlation with disease.