Instrumentation for Spectral Karyotyping

光谱核型分析是一种不断发展的细胞遗传学方法， 鉴定染色体重排的技术， 在癌细胞系中观察到的缺失、倒位和易位。一 商业仪器作为修改后的Sagnac操作 安装在落射照明显微镜上的干涉仪， 捕捉到了用于绘画的探针的光谱特征 染色体落射照明端口和荧光立方体 显微镜的组装已经过修改， 波长激发，导致大于 的信噪比的幅度改善， 荧光信号和整体背景信号的减少。 这些改进将允许分辨率较小的染色体 谱带异常及其与 疾病

Spectral karyotyping is an evolving cytogenetic technology to identify chromosomal rearrangements such as deletions, inversions, and translocations seen in cancer cell lines. A commercial instrument operates as a modified Sagnac interferometer installed on an epi-illumination microscope to capture the spectral signature of probes used to paint chromosomes. The epi-illumination port and the fluorescent cube assembly of the microscope have been modified to permit single wavelength excitation, resulting in greater than an order of magnitude improvement in the signal to noise ratio of the fluorescent signals and a reduction in the overall background signal. These improvements will permit resolution of smaller chromosome band abnormalities and identification of their correlation with disease.