Elucidating the Mechanisms of Reverse Transcription and Recombination

频繁的重组对HIV-1群体的多样性有很大贡献。我们研究了HIV-1重组的多个方面。我们使用在俄罗斯流行的毒株研究了A亚型HIV-1的重组潜力，我们发现重组经常发生，并且可以很容易地分类突变以产生对多种抗病毒药物具有抗性的变体。我们还研究了宿主限制因子APOBEC 3G和APOBEC 3F如何影响病毒复制，以及这些限制因子诱导的超突变是否在病毒进化中发挥作用。此外，我们还协助构建了可用于监测细胞间感染的复制依赖型载体。这些研究将揭示对HIV-1复制机制的见解。[对应于2011年10月艾滋病毒耐药性项目现场访问报告中的Hu项目1，2015年更名为艾滋病毒动态和复制项目]

Frequent recombination contributes significantly to the diversity of the HIV-1 population. We have studied multiple aspects of HIV-1 recombination. We have studied the recombination potential of subtype A HIV-1 using strains that are circulating in Russia, and we found that recombination occurs frequently and can easily assort mutation to generate variants resistant to multiple antivirals. We have also studied how host restriction factors APOBEC3G and APOBEC3F affect viral replication, and whether hypermutations induced by these restriction factors play a role in viral evolution. Additionally, we have assisted in the construction of replication-dependent vectors that can be used to monitor cell-to-cell infection. These studies will reveal insights into the replication mechanisms of HIV-1. [Corresponds to Hu Project 1 in the October 2011 site visit report of the HIV Drug Resistance Program, renamed the HIV Dynamics and Replication Program in 2015]