140 GHz Gyroamplifier for Time Domain DNP and EPR

用于时域 DNP 和 EPR 的 140 GHz 陀螺放大器

• 批准号: 6743712
• 负责人: RICHARD J TEMKIN
• 金额: $ 48.63万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2008-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6743712
• 关键词: biomedical equipment development; electron spin resonance spectroscopy; methane sulfonate; microwave radiation; nuclear magnetic resonance spectroscopy; quantum chemistry

DESCRIPTION (provided by applicant): We propose to design and build a 100 W, 140 GHz gyrotron amplifier and to apply it in time domain DNP/NMR and EPR experiments. During the last decade the repertoire of time domain magnetic resonance techniques that utilize high frequency microwaves in the 90-300 GHz regime has expanded considerably. Specifically, time domain EPR and DNP/NMR experiments have emerged as important techniques for elucidating the structure, function, and dynamic properties of biological systems. However, at present the full implementation of these techniques at high frequency is limited by the lack of microwave power. This proposal requests funding to develop a high power gyrotron amplifier operating at 140 GHz with a 1 GHz bandwidth and 35 dB of saturated gain. A gyrotron amplifier is particularly attractive because it is capable of extension to higher frequency. A low power (30-60 mW) phase switching and pulse forming network (with four phase and <1 ns switching capability) will be used as the input (driver) for the gyro-amplifier. This gyro-amplifier will enable the full implementation of a recently developed electron-nuclear crosspolarization (CP) experiment and the extension to high frequency of recently developed low frequency (9-18 GHz) pulsed EPR techniques for measuring long range (15-80Angstroms) electron-electron dipolar couplings in spin labeled biological systems. To summarize, the specific aims of the research can be divided into three parts: (1) Development of a 100 W, high frequency (140 GHz) gyrotron amplifier. (2) Implementation of electron-nuclear CP experiments with BDPA/PS and trityl/water glycerol samples. (3) Implementation of multiple quantum and SIFTER pulsed EPR experiments at 140 GHz using biradical spin labeled peptides.

描述(申请人提供)：我们计划设计和制造一个100W，140 GHz的回旋管放大器，并将其应用于时域DNP/核磁共振和EPR实验。在过去的十年中，在90-300 GHz范围内利用高频微波的时间域磁共振技术的曲目有了相当大的扩展。具体来说，时间域EPR和DNP/核磁共振实验已经成为阐明生物系统的结构、功能和动力学性质的重要技术。然而，目前这些技术在高频下的充分实施受到微波功率不足的限制。这项提案要求提供资金，以开发一种工作在140 GHz、带宽为1 GHz、饱和增益为35分贝的高功率回旋放大器。回旋管放大器特别吸引人，因为它能够扩展到更高的频率。一个低功率(30-60 mW)的相位切换和脉冲形成网络(具有四相和1 ns的开关能力)将被用作陀螺放大器的输入(驱动器)。这种陀螺放大器将使最近发展的电子-核交叉极化(CP)实验和最近发展的低频(9-18 GHz)脉冲EPR技术扩展到高频，以测量自旋标记生物系统中的长程(15-80埃)电子-电子偶极耦合。综上所述，研究的具体目的可以分为三个部分： (1)研制100W高频(140 GHz)回旋管放大器。 (2)对BDPA/PS和三叔丁基/水甘油样品进行了电子-核CP实验。 (3)利用双自由基自旋标记多肽实现了140 GHz的多重量子和筛选脉冲EPR实验。