MULTIDISCIPLINARY MICRO CT-3D IMAGING FACILITY

多学科微型 CT-3D 成像设备

• 批准号: 6788715
• 负责人: Erik Leo Ritman
• 金额: $ 41.45万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6788715
• 关键词: bioimaging /biomedical imaging; biomedical automation; biomedical equipment development; biomedical facility; computed axial tomography; computer program /software; human tissue; image processing; information display; kidney function; laboratory rat; radiography; renal tubule; single photon emission computed tomography

DESCRIPTION (Verbatim from Applicant's Abstract): This resubmission continues the overall goal which is to develop and apply an x-ray micro-CT scanner for generating 3-D images, of sufficient spatial resolution and specimen size, to allow quantitation of intact organ microarchitecture in terms of all Basic Functional Units (BFU, e.g., glomerulus and its nephron) as well as the transport mechanisms within the organ. The application is focused on intact rodent organs or similar-sized (1-8 cm3) "biopsies" from large animals and humans. In response to the previous review, more detail of scanner characteristics and how these relate to users of the scanner, are provided. The hypothesis of this proposal is that bench-top x-ray micro-CT can provide important new quantitative information about organ micro architecture by a combination of hardware and software enhancements motivated by their utility in meeting new biomedical research needs, such as defining the phenotype of genetically modified mice. AIM I - expand the hardware and associated software to: (a) extend the capability of our current x-ray detection device with "super"-resolution scanning modes & reconstruction algorithms and with "local" reconstruction algorithms, (b) add helical scanning capability, (c) increase x-ray flux with x-ray optics. AIM II - Image dvnamic processes bv scanning 'snap' frozen specimen: This involves snap-freezing the specimen immediately after (a) the desired distribution of contrast agent or (b) degree of mechanical distortion (e.g., inflation) has been achieved in vivo and keeping it frozen during the scan. AIM III - Quantitation of 3-D microarchitecture and associated 3-D function by image analysis via (a) extension of automated vascular "tree" finding/analysis algorithm; (b) develop a visualization tool for guiding and evaluating the automated image analysis; (c) quantitation of local strains throughout an organ that is mechanically distorted during a scan; and (d) analysis of the fluid dynamic implications of vessel branching geometry. AIM IV - Continue to provide the 3D micro-CT imaging service to investigator-users so that this experience will guide innovation, improvement and/or expansion of the imaging repertoire of the micro-CT methodology.

描述（来自申请人摘要的逐字记录）：继续重新提交 总体目标是开发和应用X射线显微CT扫描仪， 生成具有足够空间分辨率和样本尺寸的3D图像， 允许对完整器官的微结构进行定量， 功能单元（BFU，例如，肾小球及其肾单位）以及 器官内的运输机制。该应用程序的重点是完整的 啮齿类动物器官或大型动物的类似大小（1-8立方厘米）的“活组织检查”， 人类作为对上一次审查的回应， 特征以及这些特征如何与扫描仪的用户相关。的 该建议假设台式X射线显微CT可以提供 一个关于器官微结构重要的新的定量信息， 硬件和软件增强功能的组合， 满足新的生物医学研究需求，例如定义 转基因老鼠 目标一-扩展硬件和相关软件，以便： 我们目前的X射线检测设备的能力与“超”分辨率 扫描模式和重建算法以及“局部”重建 算法，（B）增加螺旋扫描能力，（c）增加X射线通量， X射线光学 AIM II -通过扫描“快速”冷冻标本的图像动态过程： 包括在（a）所需的温度升高之后立即快速冷冻样品， 造影剂的分布或（B）机械变形的程度（例如， 膨胀）已经在体内实现并且在扫描期间保持其冷冻。 目的III -定量的3-D微结构和相关的3-D功能， 通过（a）自动血管“树”发现/分析扩展的图像分析 算法;（B）开发可视化工具，用于指导和评估 自动图像分析;（c）对整个器官的局部应变进行定量 在扫描过程中机械变形;以及（d）分析流体 血管分支几何学的动态含义。 AIM IV -继续提供3D微型CT成像服务， 研究者-用户，以便这种经验将指导创新、改进 和/或扩大微CT方法的成像库。