Retroviral vector for production of transgenic chickens

描述(由申请人提供)： 到目前为止，嵌合鸡是通过将囊胚层细胞或胚胎干细胞注入X期受体胚胎而形成的，但嵌合体的频率和水平是可变的和不可预测的。我们正在开发一种贡献最少的通用接受者，这样捐赠者来源的细胞对所产生的嵌合体的贡献就会一直很高，而且是可以预测的。 在这项SBIR I期应用中，我们计划生成并评估用于生成通用受体的逆转录病毒载体。高等级的嵌合体将允许在非常短的时间内生产大量的生物制药蛋白质。当蛋白质的表达干扰鸡的繁殖能力时，高级嵌合体也将允许生产生物药用蛋白质。这一产量很容易在一年内扩大到100公斤的产量。

DESCRIPTION (provided by applicant): To date, chimeric chickens are made by injecting blastodermal cells or embryonic stem cells into stage X recipient embryos, but the frequency and level of chimerism is variable and unpredictable. We are developing a universal recipient that contributes minimally, so that the contribution of donor derived cells to the resulting chimeras is constantly high and predictable. In this SBIR phase I application we plan to generate and evaluate a retroviral vector for the generation of the universal recipient. High-grade chimeras would allow the production of significant amounts of biopharmaceutical proteins in a very short period of time. High-grade chimeras would also allow the production of biopharmaceutical protein when the expression of the protein interferes with the chicken's ability to reproduce. This production could be easily scaled up to production of 100 kg within 1 year.