Gen. of transgenic chickens using a lentiviral approach

使用慢病毒方法培育转基因鸡

• 批准号: 7105482
• 负责人: BABETTE S HEYER
• 金额: $ 78.25万
• 依托单位: ORIGEN THERAPEUTICS
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-04 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7105482
• 关键词: Lentivirus; biotechnology; cell line; chickens; embryo /fetus; gene delivery system; genetic manipulation; genetically modified animals; technology /technique development; tissue mosaicism

DESCRIPTION (provided by applicant): Currently, chimeric chickens are made by injecting blastodermal cells or chicken embryonic stem cells into stage X recipient embryos, but the frequency and the level of chimerism is variable and not predictable. We are developing a universal recipient that contributes minimally, so that the contribuion of donor dervied celld to the resulting chimera is constantly high and predictable. In phase I of this SBIR project, a lentiviral vector for the universal recipient chicken line and for a GFP control line was generated and evaluated by transducing stage X chicken embryos with a lentiviral vector. The results clearly demonstrated the feasibility of a lentiviral approach for the establishment of the universal recipient transgenic chicken lines. In phase II of this grant application, we plan to generate the universal recipient chicken lines using the lentiviral approach. The project is unique and innovative because it seeks to establish a reliable and reproducible method of compromising the recipient embryos to consistently yield high-grade chimeras and allow the production of significant amounts of protein per egg in a short period of time.

描述（由申请人提供）： 目前，嵌合鸡是通过将胚盘细胞或鸡胚胎干细胞注射到X期受体胚胎中来制造的，但嵌合的频率和水平是可变的，不可预测。我们正在开发一种贡献最小的通用接受者，这样捐赠者所获得的细胞对所产生的嵌合体的贡献就一直很高，而且是可预测的。 在该SBIR项目的I期中，通过用慢病毒载体转导X期鸡胚来产生和评估用于通用受体鸡系和GFP对照系的慢病毒载体。结果清楚地证明了慢病毒方法用于建立通用受体转基因鸡系的可行性。 在第二阶段的拨款申请中，我们计划使用慢病毒方法产生通用受体鸡品系。该项目是独特和创新的，因为它寻求建立一种可靠和可重复的方法，使受体胚胎能够持续产生高等级的嵌合体，并允许在短时间内每个卵子产生大量的蛋白质。