Optical Tomography for Cardiac Imaging of Small Animals

用于小动物心脏成像的光学断层扫描

• 批准号: 6738563
• 负责人: ROBERT A KRUGER
• 金额: $ 11.12万
• 依托单位: OPTOSONICS, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6738563
• 关键词: atherosclerosis; bioimaging /biomedical imaging; biomedical equipment development; cardiovascular imaging /visualization; laboratory mouse; optical tomography; ventricular hypertrophy

DESCRIPTION (provided by applicant): We propose to develop a thermoacoustic-imaging device capable of making axial images of the cardiac anatomy in small animals such as mice and rats using near infrared light. The device we envision will allow researchers to assess cardiac dynamics and to study transgenic models of cardiovascular disease, such as cardiac hypertrophy and atherosclerosis. Ultimately we plan to develop a device capable of forming high-resolution images at a frame rate of 100 - 200 Hz, sufficient to image the cardiac dynamics of mice and rats. One advantage offered by such a device is that cardiac dynamics can be studied without the need for injecting vascular-enhancing contrast material. In Phase I, we will design, construct and test a focused, circular transducer array capable of collecting sufficient thermoacoustic data to reconstruct a 1-mm-thick axial slice following a single, 10-ns pulse of near infrared laser light. Because the laser pulse is so short, we should encounter virtually no blur due to heart-wall motion when imaging live animals. We hope to achieve in-plane spatial resolution of < 200 microns. Due to limitations of our present digital acquisition system (DAS), we will only have the capability of forming images at 2 frames per second during Phase I. In Phase II we will demonstrate our ability to form dynamic image sequences of the heart in mice and rats. To this end we will redesign our DAS to allow us to acquire dynamic image sequences at rates as high as 200 Hz. We will also incorporate a custom-designed, tunable, near-infrared laser capable of pulsing at 200 Hz. In this phase we will develop multi-slice imaging protocols to form 3-D images of the beating heart.

描述（由申请人提供）： 我们建议开发一种热声成像设备，能够使用近红外光在小动物，如小鼠和大鼠的心脏解剖结构的轴向图像。我们设想的设备将允许研究人员评估心脏动力学和研究心血管疾病的转基因模型，如心脏肥大和动脉粥样硬化。最终，我们计划开发一种能够以100 - 200 Hz的帧速率形成高分辨率图像的设备，足以对小鼠和大鼠的心脏动力学进行成像。这种装置提供的一个优点是可以研究心脏动力学而不需要注射血管增强造影剂。 在第一阶段，我们将设计，建造和测试一个集中的，圆形的换能器阵列能够收集足够的热声数据，重建一个1毫米厚的轴向切片后，一个单一的，10纳秒脉冲的近红外激光。由于激光脉冲非常短，我们在对活体动物成像时几乎不会遇到由于心壁运动而造成的模糊。我们希望实现< 200微米的面内空间分辨率。由于我们现时的数码采集系统的限制，我们在第一阶段只能以每秒2帧的速度成像。 在第二阶段，我们将证明我们的能力，形成动态图像序列的心脏在小鼠和大鼠。为此，我们将重新设计DAS，使我们能够以高达200 Hz的速率采集动态图像序列。我们还将采用定制设计的可调谐近红外激光器，能够以200 Hz的频率脉冲。在这一阶段，我们将开发多层面成像协议，以形成跳动心脏的3-D图像。