In Vivo Immunofluorescence Microscopy and Cytometry

体内免疫荧光显微镜和细胞计数

• 批准号: 6940609
• 负责人: Charles P. Lin
• 金额: $ 42.85万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-15 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6940609
• 关键词: antibody; biomarker; blood flow measurement; cell migration; cell sorting; cellular immunity; confocal scanning microscopy; cytology; dendritic cells; flow cytometry; fluorescence microscopy; imaging /visualization /scanning; leukocytes; method development; molecular /cellular imaging; tumor necrosis factor alpha; vascular endothelium

DESCRIPTION (provided by applicant): The overall long-term goal of this multidisciplinary research program is to apply new optical technology to investigate early changes associated with cellular immune response and to monitor response to therapy over time in vivo and at the cellular level. Specifically, we proposed to develop in vivo immunofluorescence microscopy for imaging specific cell surface markers expressed by vascular and lymphatic endothelial cells, circulating leukocytes, and tissue dendritic cells. The technology that enables noninvasive, real-time cellular imaging is a video rate confocal and multiphoton fluorescence microscope that we recently developed in our laboratory. We will apply this technology to study important steps involved in the regulation of cellular immune response in vivo, including endothelial cell activation, leukocyteendothelial interaction, and dendritic cell recruitment and migration. In addition, we proposed to develop an in vivo flow cytometer, a novel technology for real-time, noninvasive detection and quantification of circulating cells that are tagged with fluorescent antibodies or antibody fragments. We will use this system to monitor changes in specific T cell population in response to immunomodulation, to detect circulating tumor cells and to investigate the correlation between tumor cell shedding and metastatic potential.

描述（由申请人提供）： 该多学科研究计划的总体长期目标是应用新的光学技术来研究与细胞免疫反应相关的早期变化，并监测体内和细胞水平上随时间推移对治疗的反应。具体来说，我们建议开发体内免疫荧光显微镜，用于对血管和淋巴内皮细胞、循环白细胞和组织树突细胞表达的特定细胞表面标记物进行成像。实现无创实时细胞成像的技术是我们最近在实验室开发的视频速率共焦和多光子荧光显微镜。我们将应用该技术来研究体内细胞免疫反应调节的重要步骤，包括内皮细胞激活、白细胞内皮相互作用以及树突状细胞招募和迁移。此外，我们建议开发一种体内流式细胞仪，这是一种实时、无创检测和定量标记有荧光抗体或抗体片段的循环细胞的新技术。我们将使用该系统监测特定 T 细胞群响应免疫调节的变化，检测循环肿瘤细胞并研究肿瘤细胞脱落与转移潜力之间的相关性。