2004 Biophysical Discussion: Membrane Microdomains

2004 年生物物理讨论：膜微域

• 批准号: 6834500
• 负责人: Kenneth A Jacobson
• 金额: $ 1万
• 依托单位: BIOPHYSICAL SOCIETY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6834500
• 关键词: X ray crystallography; biophysics; computational biology; electron microscopy; mass spectrometry; meeting /conference /symposium; membrane activity; membrane model; membrane structure; nuclear magnetic resonance spectroscopy; travel

DESCRIPTION (provided by applicant): The 2004 Biophysical Discussions will be on the topic, "Probing membrane microdomains." The meeting will be held in Asilomar, Monterey, CA from Oct. 28-31, 2004. All speakers will be describing techniques, including theoretical methods that can be used, in principle, to investigate lipid and protein lateral heterogeneity in membranes--a topic of commanding interest with the advent of the notion of rafts. The putative rafts are of basic interest to biophysicists studying membrane lateral organization and to biologists who need to know the functional consequences of lateral organization. However, the raft field is at a technical impasse because there is no unambiguous structural correlate of the biochemically defined raft. Different methods give different answers. This is because the membrane is an ordered two-dimensional liquid for which we are still developing the appropriate means of investigation. This Discussion will therefore focus on the currently available techniques that can be used to characterize membrane lateral heterogeneity--their advantages and limitations. These techniques include fluorescence and electron microscopic methods, NMR, mass spec. and X-ray studies, as well computational studies. Applications will include those focused both on model membranes and cell membranes. The meeting will also consider newly available techniques and what types of methodology should be developed in the future. The format of the Biophysical Discussions is unique in its stress on discussion and prior preparation by the participants.

描述(申请人提供)：2004年的生物物理学讨论将以“探测膜微域”为主题。会议将于2004年10月28日至31日在加利福尼亚州蒙特利的阿西洛马市举行。所有发言者都将描述技术，包括原则上可用于研究膜中脂质和蛋白质横向异质性的理论方法--随着筏子概念的出现，这是一个令人感兴趣的话题。对于研究膜横向组织的生物物理学家和需要知道横向组织的功能后果的生物学家来说，假定的筏子是基本感兴趣的。然而，由于生物化学定义的浮筏没有明确的结构相关性，因此浮筏领域处于技术僵局。不同的方法给出了不同的答案。这是因为膜是一种有序的二维液体，我们仍在为其开发适当的研究手段。因此，本讨论将集中在目前可用来表征膜横向异质性的技术上--它们的优点和局限性。这些技术包括荧光和电子显微镜方法、核磁共振、质谱学。和X射线研究，以及计算研究。应用领域包括模型膜和细胞膜。会议还将审议新的可用技术以及今后应开发哪些类型的方法。生物物理讨论的形式是独特的，因为它侧重于与会者的讨论和事先准备。