2004 Biophysical Discussion: Membrane Microdomains

2004 年生物物理讨论：膜微域

• 批准号: 6834500
• 负责人: Kenneth A Jacobson
• 金额: $ 1万
• 依托单位: BIOPHYSICAL SOCIETY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6834500
• 关键词: X ray crystallography; biophysics; computational biology; electron microscopy; mass spectrometry; meeting /conference /symposium; membrane activity; membrane model; membrane structure; nuclear magnetic resonance spectroscopy; travel

DESCRIPTION (provided by applicant): The 2004 Biophysical Discussions will be on the topic, "Probing membrane microdomains." The meeting will be held in Asilomar, Monterey, CA from Oct. 28-31, 2004. All speakers will be describing techniques, including theoretical methods that can be used, in principle, to investigate lipid and protein lateral heterogeneity in membranes--a topic of commanding interest with the advent of the notion of rafts. The putative rafts are of basic interest to biophysicists studying membrane lateral organization and to biologists who need to know the functional consequences of lateral organization. However, the raft field is at a technical impasse because there is no unambiguous structural correlate of the biochemically defined raft. Different methods give different answers. This is because the membrane is an ordered two-dimensional liquid for which we are still developing the appropriate means of investigation. This Discussion will therefore focus on the currently available techniques that can be used to characterize membrane lateral heterogeneity--their advantages and limitations. These techniques include fluorescence and electron microscopic methods, NMR, mass spec. and X-ray studies, as well computational studies. Applications will include those focused both on model membranes and cell membranes. The meeting will also consider newly available techniques and what types of methodology should be developed in the future. The format of the Biophysical Discussions is unique in its stress on discussion and prior preparation by the participants.

描述（由申请人提供）：2004 年生物物理讨论的主题是“探测膜微域”。会议将于 2004 年 10 月 28 日至 31 日在加利福尼亚州蒙特利的阿西洛玛举行。所有发言者都将描述技术，包括原则上可用于研究膜中脂质和蛋白质横向异质性的理论方法——随着筏概念的出现，这是一个引起人们极大兴趣的话题。假定的筏对于研究膜横向组织的生物物理学家和需要了解横向组织的功能后果的生物学家来说具有基本的兴趣。然而，筏领域处于技术僵局，因为没有生化定义的筏的明确结构关联。不同的方法给出不同的答案。这是因为膜是一种有序的二维液体，我们仍在开发适当的研究方法。因此，本讨论将重点关注当前可用的可用于表征膜横向异质性的技术——它们的优点和局限性。这些技术包括荧光和电子显微镜方法、NMR、质谱法。 X 射线研究，以及计算研究。应用将包括那些同时关注模型膜和细胞膜的应用。会议还将考虑新的可用技术以及未来应开发哪些类型的方法。生物物理讨论的形式独特，强调参与者的讨论和事先准备。