Imaging/Photomanipulation

成像和光操纵倡议（I&PI）的目标是开发技术， 测量和干扰介导和调节细胞迁移的局部活性，并使用这些 技术，使定量测量建模细胞迁移。新兴的实验 一个范例是最初检测和量化迁移细胞中的局部分子事件，然后操纵这种分子事件。 事件的局部激活或失活，并评估对迁移过程的后果。主动 在开发实现这一目标的化学和物理技术以及试剂方面取得了很大进展 范例下一个资助期的目标不仅是继续开发这些技术， 还将它们用于原型迁移现象，以开发研究细胞迁移的工作模式。 这些过程在空间和时间上都受到严格的调控。I&PI的目标是：（1）开发 在细胞培养中，在微模式细胞培养中，以及在组织中，测试新的生物传感器和探针， 光活化和局部光灭活，使用生色团辅助激光灭活（卡利）;（2）继续 开发相关方法，用于测量分子相互作用、浓度和动力学， 体外和组织中;以及，（3）利用目标（1）和（2）中提供的进展来开发有用的数据 由联盟内外的研究人员对迁移现象进行建模。

The goal of the Imaging and Photomanipulation Initiative (I&PI) is to develop technologies for measuring and perturbing the localized activities that mediate and regulate cell migration and to use these technologies to make quantitative measurements for modeling cell migration. The emerging experimental paradigm is to initially detect and quantify local molecular events in migrating cells and then to manipulate such events by local activation or inactivation and assess the consequences on the migratory process. The Initiative has made great progress in developing the chemical and physical technologies and reagents to implement this paradigm. The goals for the next granting period are not only to continue developing these technologies, but also to use them on prototypical migration phenomena to develop working paradigms for studying cellular processes that are tightly regulated both spatially and temporally. The objectives of the I&PI are to: (1) develop and test, in cell cultures, in micro-patterned cell cultures, and tissues, new biosensors and probes both for local photoactivation and local photoinactivation, using chromophore assisted laser inactivation (CALI); (2) continue development of correlation methods for measuring molecular interactions, concentrations and dynamics both in vitro and in tissues; and, (3) use the advances provided in objectives (1) and (2) to develop data useful for modeling migration phenomena by investigators both inside and outside the Consortium.