Imaging/Photomanipulation

成像和光操纵倡议(I&PI)的目标是开发 测量和干扰调节和调节细胞迁移的局部活动，并使用这些 为模拟细胞迁移而进行定量测量的技术。新兴的实验 范式是最初检测和量化迁移细胞中的局部分子事件，然后操纵这种 通过当地激活或停用事件，并评估对移徙进程的后果。《倡议》 在开发化学和物理技术和试剂以实现这一点方面取得了很大进展 范例。下一个授权期的目标不仅是继续开发这些技术，而且 也将它们用于研究典型的迁移现象，以开发研究细胞的工作范例 在空间和时间上都受到严格控制的过程。I&PI的目标是：(1)发展 并在细胞培养、微图案化细胞培养和组织中测试新的生物传感器和探针，用于局部 光活化和局部光灭活，使用生色团辅助激光灭活(CALI)；(2)继续 测量分子间相互作用、浓度和动力学的相关方法的发展 以及，(3)使用目标(1)和(2)中提供的进展来开发有用的数据 由联合体内外的调查人员模拟迁徙现象。

The goal of the Imaging and Photomanipulation Initiative (I&PI) is to develop technologies for measuring and perturbing the localized activities that mediate and regulate cell migration and to use these technologies to make quantitative measurements for modeling cell migration. The emerging experimental paradigm is to initially detect and quantify local molecular events in migrating cells and then to manipulate such events by local activation or inactivation and assess the consequences on the migratory process. The Initiative has made great progress in developing the chemical and physical technologies and reagents to implement this paradigm. The goals for the next granting period are not only to continue developing these technologies, but also to use them on prototypical migration phenomena to develop working paradigms for studying cellular processes that are tightly regulated both spatially and temporally. The objectives of the I&PI are to: (1) develop and test, in cell cultures, in micro-patterned cell cultures, and tissues, new biosensors and probes both for local photoactivation and local photoinactivation, using chromophore assisted laser inactivation (CALI); (2) continue development of correlation methods for measuring molecular interactions, concentrations and dynamics both in vitro and in tissues; and, (3) use the advances provided in objectives (1) and (2) to develop data useful for modeling migration phenomena by investigators both inside and outside the Consortium.