Investigating the role of TRP channels in the pathogenesis of multiple sclerosis

研究问题：与全局TRPA1-/-小鼠相比，少突胶质细胞中的TRPA1通道是否调节胶质功能，导致条件性TRPA1-/-小鼠较少的脱髓鞘和更多的重新髓鞘形成？假设：表达在少突胶质细胞上的TRPA1通道调节神经胶质功能，并在脱髓鞘疾病期间变得功能障碍；在啮齿类丘脑地带模型中，少突胶质细胞中的TRPA1-/-对脱髓鞘具有保护作用。目的1：验证少突胶质细胞中条件性TRPA1-/-与全局TRPA1-/-小鼠的比较。目的2：通过使用铜必宗模型，确定条件TRPA1-/-与WTT的脱髓鞘程度。目的3：通过使用Cuprizone模型，确定条件TRPA1-/-VS WT的再髓鞘程度

Research Question: Does oligodendrocyte TRPA1 channel regulate glial functioning and lead to less demyelination and increased remyelination in conditional TRPA1 -/-in oligodendrocytes compared to global TRPA1 -/-mice?Hypothesis:TRPA1 channels expressed on oligodendrocyte regulate glial functioning and become dysfunctional during demyelinating disease; TRPA1 -/-in oligodendrocytes is protective against demyelinationin the rodent cuprizone model. Aim 1:Validate the conditional TRPA1 -/-in oligodendrocyte compared to global TRPA1 -/-mice. Aim 2:Through use of the cuprizone model, determine the extent of demyelination in conditional TRPA1 -/-vs WTAim 3:Through use of the cuprizone model, determine the extent of remyelination in conditional TRPA1 -/-vs WT