Information Coding in the Olfactory Cortex

嗅觉皮层的信息编码

• 批准号: 6866388
• 负责人: JAMES J CONTOS
• 金额: $ 5.35万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6866388
• 关键词: alcohols; amines; brain mapping; calcium; chemoreceptors; gene expression; gene targeting; genetically modified animals; green fluorescent proteins; imaging /visualization /scanning; immunocytochemistry; laboratory mouse; lectin; neural information processing; odors; olfactions; olfactory lobe; polymerase chain reaction; postdoctoral investigator; protein protein interaction; protein structure function; sensory mechanism; single cell analysis; thiols

DESCRIPTION (provided by applicant): The research proposed here will define further how odor information from peripheral olfactory sensory neurons is encoded by odorant receptors (ORs) and also how it is organized as it progresses to the olfactory cortex (OC). A screen will be performed to identify additional ORs that recognize thiols and structurally similar odorants with highly divergent qualitative features (e.g. hexanethiol, which has a foul odor, and hexanol, which has a sweet smell). This will be accomplished with dissociated mouse olfactory sensory neurons using Ca++ imaging and single cell PCR. ORs of similar and divergent sequences recognizing the same odorant will be targeted with knockin constructs, resulting in coexpression of differentially tagged transneuronal tracers (barley lectin). Areas of the OC containing BL will be determined with immunohistochemistry in both single and double knockin animals. This will allow determination of the overlap of multiple OR inputs to the OC. In addition to helping illuminate how OR structure is related to odorant detection, these experiments will determine whether OR inputs to the OC are organized by function and/or structure of the OR.

描述（由申请人提供）：本文提出的研究将进一步定义来自周围嗅觉感觉神经元的气味信息是如何被气味受体（ORs）编码的，以及它是如何在进入嗅觉皮层（OC）时被组织起来的。将进行筛选，以识别识别具有高度不同定性特征的硫醇和结构相似的气味剂（例如具有恶臭气味的己硫醇和具有甜味的己醇）的其他or。这将通过Ca++成像和单细胞PCR分离小鼠嗅觉感觉神经元来完成。识别相同气味的相似和不同序列的or将被敲除蛋白靶向，导致差异标记的跨神经元示踪剂（大麦凝集素）的共表达。在单敲和双敲动物中，用免疫组织化学方法确定含有BL的OC区域。这将允许确定多个OR输入到OC的重叠。除了帮助阐明OR结构如何与气味检测相关之外，这些实验将确定OC的OR输入是否由OR的功能和/或结构组织。