Information Coding in the Olfactory Cortex

嗅觉皮层的信息编码

• 批准号: 6791667
• 负责人: JAMES J CONTOS
• 金额: $ 5.05万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6791667
• 关键词: alcohols; amines; brain mapping; calcium; chemoreceptors; gene expression; gene targeting; genetically modified animals; green fluorescent proteins; imaging /visualization /scanning; immunocytochemistry; laboratory mouse; lectin; neural information processing; odors; olfactions; olfactory lobe; polymerase chain reaction; postdoctoral investigator; protein protein interaction; protein structure function; sensory mechanism; single cell analysis; thiols

DESCRIPTION (provided by applicant): The research proposed here will define further how odor information from peripheral olfactory sensory neurons is encoded by odorant receptors (ORs) and also how it is organized as it progresses to the olfactory cortex (OC). A screen will be performed to identify additional ORs that recognize thiols and structurally similar odorants with highly divergent qualitative features (e.g. hexanethiol, which has a foul odor, and hexanol, which has a sweet smell). This will be accomplished with dissociated mouse olfactory sensory neurons using Ca++ imaging and single cell PCR. ORs of similar and divergent sequences recognizing the same odorant will be targeted with knockin constructs, resulting in coexpression of differentially tagged transneuronal tracers (barley lectin). Areas of the OC containing BL will be determined with immunohistochemistry in both single and double knockin animals. This will allow determination of the overlap of multiple OR inputs to the OC. In addition to helping illuminate how OR structure is related to odorant detection, these experiments will determine whether OR inputs to the OC are organized by function and/or structure of the OR.

描述（由申请人提供）：此处提出的研究将进一步定义来自外周嗅觉感觉神经元的气味信息如何由气味受体（OR）编码，以及它在进入嗅觉皮层（OC）时如何组织。将进行筛选以识别其他 OR，这些 OR 可以识别硫醇和结构相似的气味剂，但具有高度不同的定性特征（例如，具有恶臭气味的己硫醇和具有甜味的己醇）。这将通过使用 Ca++ 成像和单细胞 PCR 分离小鼠嗅觉感觉神经元来完成。识别相同气味的相似和不同序列的 OR 将被敲入构建体靶向，从而导致差异标记的跨神经元示踪剂（大麦凝集素）的共表达。含有 BL 的 OC 区域将通过单敲入和双敲入动物的免疫组织化学测定。这将允许确定 OC 的多个 OR 输入的重叠。除了帮助阐明 OR 结构如何与气味检测相关之外，这些实验还将确定 OC 的 OR 输入是否按 OR 的功能和/或结构进行组织。