Array-microscope slide scanner system

阵列显微镜载玻片扫描仪系统

• 批准号: 6934904
• 负责人: ARTUR G OLSZAK
• 金额: $ 63.53万
• 依托单位: DMETRIX, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-07 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6934904
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; computer program /software; data management; digital imaging; high throughput technology; histopathology; image processing; microscopy; miniature biomedical equipment; optics

DESCRIPTION (PROVIDED BY APPLICANT): We propose the development of an innovative, economical, array-microscope digital slide scanner to generate virtual slides in histopathology. This instrument will be capable of scanning a microscope slide in a single sweep at a numerical aperture of NA = 0.65, covering the entire width and length of a slide with 0.47- um sampling. The fundamental innovation consists in the use of an array of 100 miniaturized objectives, each measuring approximately 2 mm in diameter and 9 mm in length, slightly staggered in the array, to produce a single, seamless sweep of the slide. This innovation allows a very rapid full-color scan requiring less than 60 seconds for a full microscope slide of 20 mm by 50 mu m, at diffraction-limited sampling. This scanning speed is a factor of 10-20 faster than any single-objective system's. The Phase I application laid out the scientific innovation and merit of the array microscope scanning technology. It is the overall goal of Phase II to build on the accomplishments of Phase I and produce an economical, high-throughput digital slide scanner that can be either a stand-alone solution or integrated with existing image-management and analysis solutions. We will achieve this goal through a combination of developments in manufacturing (result: economical scanner) and software (result: tools to seamlessly connect our instrument to third-party laboratory-information-system or quantitative-analysis software). The three Aims of Phase II are: (Aim 1) Develop the means to manufacture high-numerical-aperture array microscope optics at minimal cost. Specifically, implement and perfect injection-molding of high-precision optical elements arranged as arrays on easy-to-assemble, coin-sized plates; (Aim 2) Develop the methods necessary to quality-control (QC) arrays of miniature microscope objectives; and (Aim 3) Develop a Software Development Kit (SDK) to allow third-party developers to create clinical and research solutions that combine DMetrix's array microscope instrument and specialized software.

描述（由申请人提供）：我们建议开发一种创新的，经济的，阵列显微镜数字切片扫描仪来生成组织病理学中的虚拟切片。该仪器将能够在NA = 0.65的数值孔径下单次扫描显微镜载玻片，以0.47- um采样覆盖载玻片的整个宽度和长度。最根本的创新在于使用一个由100个小型化物镜组成的阵列，每个物镜直径约2毫米，长度约9毫米，在阵列中稍微交错，以产生一个单一的，无缝扫描的载玻片。这一创新使得在衍射有限的采样条件下，对20毫米× 50毫米的全显微镜载玻片进行快速全彩色扫描所需时间不到60秒。这种扫描速度比任何单目标系统都快10-20倍。