Array-microscope slide scanner system

阵列显微镜载玻片扫描仪系统

• 批准号: 6934904
• 负责人: ARTUR G OLSZAK
• 金额: $ 63.53万
• 依托单位: DMETRIX, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-07 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6934904
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; computer program /software; data management; digital imaging; high throughput technology; histopathology; image processing; microscopy; miniature biomedical equipment; optics

DESCRIPTION (PROVIDED BY APPLICANT): We propose the development of an innovative, economical, array-microscope digital slide scanner to generate virtual slides in histopathology. This instrument will be capable of scanning a microscope slide in a single sweep at a numerical aperture of NA = 0.65, covering the entire width and length of a slide with 0.47- um sampling. The fundamental innovation consists in the use of an array of 100 miniaturized objectives, each measuring approximately 2 mm in diameter and 9 mm in length, slightly staggered in the array, to produce a single, seamless sweep of the slide. This innovation allows a very rapid full-color scan requiring less than 60 seconds for a full microscope slide of 20 mm by 50 mu m, at diffraction-limited sampling. This scanning speed is a factor of 10-20 faster than any single-objective system's. The Phase I application laid out the scientific innovation and merit of the array microscope scanning technology. It is the overall goal of Phase II to build on the accomplishments of Phase I and produce an economical, high-throughput digital slide scanner that can be either a stand-alone solution or integrated with existing image-management and analysis solutions. We will achieve this goal through a combination of developments in manufacturing (result: economical scanner) and software (result: tools to seamlessly connect our instrument to third-party laboratory-information-system or quantitative-analysis software). The three Aims of Phase II are: (Aim 1) Develop the means to manufacture high-numerical-aperture array microscope optics at minimal cost. Specifically, implement and perfect injection-molding of high-precision optical elements arranged as arrays on easy-to-assemble, coin-sized plates; (Aim 2) Develop the methods necessary to quality-control (QC) arrays of miniature microscope objectives; and (Aim 3) Develop a Software Development Kit (SDK) to allow third-party developers to create clinical and research solutions that combine DMetrix's array microscope instrument and specialized software.

描述（由申请人提供）：我们建议开发一种创新、经济的阵列显微镜数字载玻片扫描仪，以生成组织病理学中的虚拟载玻片。该仪器能够以 NA = 0.65 的数值孔径单次扫描显微镜载玻片，以 0.47 微米的采样覆盖载玻片的整个宽度和长度。基本创新在于使用由 100 个微型物镜组成的阵列，每个物镜的直径约为 2 毫米，长度约为 9 毫米，在阵列中稍微交错，以产生单一、无缝的载玻片扫描。这项创新可实现非常快速的全色扫描，在衍射极限采样时，20 毫米 x 50 微米的完整显微镜载玻片只需不到 60 秒。该扫描速度比任何单目标系统快 10-20 倍。 一期应用展示了阵列显微镜扫描技术的科学创新和优点。第二阶段的总体目标是在第一阶段所取得的成就的基础上，生产出经济、高通量的数字切片扫描仪，该扫描仪可以是独立的解决方案，也可以与现有的图像管理和分析解决方案集成。我们将通过制造（结果：经济型扫描仪）和软件（结果：将我们的仪器无缝连接到第三方实验室信息系统或定量分析软件的工具）的发展相结合来实现这一目标。 第二阶段的三个目标是：（目标 1）开发以最低成本制造高数值孔径阵列显微镜光学器件的方法。具体来说，在易于组装的硬币大小的板上实现并完美注射成型高精度光学元件阵列； （目标 2）开发微型显微镜物镜质量控制 (QC) 阵列所需的方法； （目标 3）开发软件开发套件 (SDK)，以允许第三方开发人员创建结合 DMetrix 阵列显微镜仪器和专用软件的临床和研究解决方案。