Miniaturized objective-lens array for slide scanner

用于载玻片扫描仪的小型化物镜阵列

• 批准号: 6642968
• 负责人: ARTUR G OLSZAK
• 金额: $ 10.7万
• 依托单位: DMETRIX, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-07 至 2004-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6642968
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; digital imaging; histopathology; image processing; microscopy; miniature biomedical equipment; optics

DESCRIPTION (provided by applicant): We propose development of innovative optics for a slide scanner to generate virtual slides in histopathology. These optics will allow scanning a microscope slide in a single sweep at a numerical aperture of NA = 0.65, covering the entire width and length of a slide with 0.5-mu sampling. The innovation consists of the use of an array of miniaturized objectives, each measuring approximately 2 mm in diameter and 10 mm in length, slightly staggered in the array, to produce a single, seamless sweep of the slide. The proposed innovation allows very rapid scanning, requiring less than 60 seconds for a full microscope slide of 20 mm x 50 mm, at diffraction-limited sampling. Specialized diagnostic expertise is expensive and frequently difficult to find. Therefore, materials are increasingly evaluated by telepathology, i.e., by sending digitized imagery from a remote location to a centralized diagnostic facility. Unfortunately, scanning of histopathologic slides is still very time-consuming. The parallelization of image acquisition by means of the array microscope results in a gain in scanning speed by a factor of 15-20 over any single-objective system. Furthermore, the array-microscope solution reduces the computational burden that is required in stitching of image tiles or swaths used in conventional scanning systems. The innovation further resides in an optics design based on injection-molded plastic aspheric lens elements, generated in large numbers on separate plates for each element. By stacking up the plates bearing the lens elements for the objectives, the array of microscope objectives is assembled fully in parallel.

描述（由申请人提供）：我们建议开发用于载玻片扫描仪的创新光学器件，以生成组织病理学中的虚拟载玻片。这些光学器件将允许以 NA = 0.65 的数值孔径单次扫描显微镜载玻片，以 0.5-mu 采样覆盖载玻片的整个宽度和长度。该创新包括使用一系列微型物镜，每个物镜的直径约为 2 毫米，长度为 10 毫米，在阵列中稍微交错，以产生单一、无缝的载玻片扫描。所提出的创新技术可实现非常快速的扫描，在衍射极限采样时，扫描 20 毫米 x 50 毫米的完整显微镜载玻片只需不到 60 秒。 专业的诊断专业知识价格昂贵，而且往往很难找到。 因此，材料越来越多地通过远程病理学进行评估，即通过将数字化图像从远程位置发送到集中诊断设施。不幸的是，组织病理学载玻片的扫描仍然非常耗时。 通过阵列显微镜进行图像采集的并行化使得扫描速度比任何单物镜系统提高 15-20 倍。此外，阵列显微镜解决方案减少了传统扫描系统中使用的图像块或条带拼接所需的计算负担。 该创新还在于基于注塑塑料非球面透镜元件的光学设计，每个元件在单独的板上大量生成。通过堆叠带有物镜透镜元件的板，显微镜物镜阵列完全平行组装。