Liquid Lens Array Microscope

液体透镜阵列显微镜

• 批准号: 7325310
• 负责人: ARTUR G OLSZAK
• 金额: $ 10.57万
• 依托单位: DMETRIX, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7325310
• 关键词: Adopted; Area; Arizona; Biological; Caliber; Cells; Clinical; Clinical Pathology; Clinical Research; Computer software; Computer-Assisted Diagnosis; Coupled; Cytology; Cytology Histology; Cytopathology; Data; Depth; Development; Device or Instrument Development; Devices; Diagnostic; Diamond; Dimensions; Electrodes; Elements; Ensure; Environmental Monitoring; Evaluation; Freezing; Glass; Goals; Healthcare; Hour; Image; Image Analysis; Imaging Device; Individual; Information Technology; Lasers; Length; Liquid substance; Marketing; Measurement; Measures; Meniscus structure of joint; Metric; Microscope; Microscopic; Microscopy; Modality; Optics; Pathology; Performance; Phase; Plant Roots; Pliability; Principal Investigator; Printing; Purpose; Rate; Refractive Indices; Relative (related person); Reporting; Research; Resolution; Sampling; Scanning; Shapes; Slide; Solutions; Specimen; Speed; Staging; Surface; System; Techniques; Technology; Telecommunications; Telemedicine; Testing; Thick; Time; Tissue Microarray; Tissues; Today; Translating; Translations; Universities; Width; base; cost; design; detector; digital; digital imaging; drug development; electric field; experience; fluorescence imaging; indexing; instrument; interest; lens; liquid crystal; miniaturize; next generation; novel; performance tests; polarized light; pre-clinical; prognostic; programs; prototype; scale up; submicron; trend; virtual; voltage

DESCRIPTION (provided by applicant): We propose to develop a next-generation, highly flexible optical system for use in a whole- slide scanner to rapidly generate virtual slides in pathology and cytology. The primary use of this optical system is to enable ultra-rapid and accurate imaging of tissue, frozen-tissue, tissue-microarray (TMA), and cytology glass slides for clinical and research purposes. This optical system will allow scanning a microscope slide in a single sweep at a minimum numerical aperture of NA = 0.65, covering the entire width and length of a glass slide with 0.23-¿m sampling. The optical system is based on an array of miniature microscopes. Each microscope in the array includes a variable-optical-power, liquid-lens element. The liquid-lens elements are controlled electrically and permit the individual focusing of each miniature microscope during scanning of tissue and cell specimens. As a result, the array microscope can rapidly and continually reconfigure itself during imaging to produce the most accurate, high-quality images of histology and cytology specimens. The long-term goal is to image slide-borne specimens in less than 60 seconds at 0.23-¿m sampling (i.e., equivalent to a 40X magnification). The proposed liquid lenses are designed to rapidly adjust focus or to enable extended-depth-of-field imaging relevant for cytology slides. The Phase I project emphasizes development of a novel liquid-lens element and assembly of a liquid-lens array microscope prototype. The evaluation of the prototype emphasizes image quality as a function of focus at multiple depths achieved with the liquid lens. Image quality will be measured in terms of resolution and distortion. In Phase II, we plan to complete development of a modular, "industrial" version of the liquid lens array-microscope instrument including 120 miniature microscopes. Liquid-lens technology, combined with DMetrix's ultra-rapid, automated array-microscope technology, represents a key development in advancing diagnostic and prognostic tissue-based digital-pathology analysis. The result of this combination will be a less costly, fast and highly accurate microscopic imaging device. This device will generate high-resolution digital images for use in telemedicine, reduced turnaround-time healthcare, drug development, and computer-aided diagnosis.

描述(由申请人提供)：我们建议开发一种新一代、高度灵活的光学系统，用于全幻灯片扫描仪，以快速生成病理学和细胞学的虚拟幻灯片。该光学系统的主要用途是为临床和研究目的实现对组织、冷冻组织、组织微阵列(TMA)和细胞学玻片的超快速和准确成像。该光学系统将允许以最小数值孔径NA=0.65的单次扫描扫描显微镜载玻片，以0.23？m的采样覆盖整个载玻片的宽度和长度。该光学系统是基于一系列微型显微镜。阵列中的每个显微镜都包括一个可变光学倍数的液体透镜元件。液体透镜元件是电子控制的，在扫描组织和细胞样本时允许每个微型显微镜单独对焦。因此，阵列显微镜可以在成像过程中快速、连续地自我重新配置，以产生组织学和细胞学标本的最准确、高质量的图像。长期目标是在0.23米的采样条件下，在不到60秒的时间内对载玻片上的标本进行成像(即相当于放大40倍)。拟议的液体透镜旨在快速调整焦距或实现与细胞学幻灯片相关的扩展景深成像。第一阶段的项目重点是开发一种新型的液体透镜元件和组装液体透镜阵列显微镜原型。对样机的评估强调图像质量是通过液体透镜实现的多个深度的焦距的函数。图像质量将以分辨率和失真来衡量。在第二阶段，我们计划完成液体透镜阵列显微镜仪器的模块化、工业版的开发，其中包括120台微型显微镜。液体透镜技术与DMetrix的超快速自动阵列显微镜技术相结合，代表着在推进基于组织的诊断和预后数字病理分析方面的关键发展。这种结合的结果将是一种成本更低、速度更快、精度更高的显微成像设备。该设备将生成高分辨率数字图像，用于远程医疗、减少周转时间的医疗保健、药物开发和计算机辅助诊断。