Charge-based Pathogen DNA Detection

基于电荷的病原体 DNA 检测

• 批准号: 6901070
• 负责人: Ronald Wayne Davis
• 金额: $ 31.69万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-15 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6901070
• 关键词: Bacillus subtilis; DNA; DNA binding protein; bacterial DNA; bacterial proteins; bioassay; biomaterial development /preparation; biomaterial evaluation; biosensor device; biotechnology; electrodes; electron microscopy; heavy metals; method development; nucleic acid probes; nucleic acid quantitation /detection; nucleic acid sequence; polymerization; surface property

DESCRIPTION (provided by applicant): Rapid and sensitive detection of biological pathogens in the environment is an essential capability in many areas, including basic research, health care, environmental monitoring, and homeland defense. This detection is often done through molecular diagnostics, but current technology limits the capability of identifying the appropriate agents present in a sample in a fast and sensitive manner. The Stanford Genome technology Center has developed and performed preliminary experiments with a new method for detecting and identifying nucleic acid based targets at extremely low concentrations. This method, Charge-Perturbation Signature (CPS), is a highly sensitive, specific and rapid method for detecting the presence of a specific sequence of DNA. Sensitivity is in the low femtomol range, which suggests no need for PCR amplification. In this project, Stanford will further develop and improve CPS and demonstrate its application to pathogen DNA detection in a proof-of-principle experiment.

描述（由申请人提供）：在许多领域，包括基础研究，卫生保健，环境监测和国土防御在内，对环境中生物病原体的快速和敏感检测是重要的能力。这种检测通常是通过分子诊断来完成的，但是当前的技术限制了以快速和敏感的方式识别样品中适当的药物的能力。斯坦福基因组技术中心已经开发并进行了初步实验，该实验是一种新方法，用于检测和识别极低浓度的基于核酸的靶标。该方法是电荷扰动特征（CPS），是一种高度敏感，特异性和快速的方法，用于检测特定的DNA序列的存在。灵敏度在低FEMTOMOL范围内，这表明不需要PCR扩增。在这个项目中，斯坦福大学将进一步发展和改善CPS，并在原理证明实验中证明其在病原体DNA检测中的应用。