权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

A nanophotonic approach to building DNA using enzymatic synthesis

使用酶合成构建 DNA 的纳米光子方法

基本信息

批准号：
10705040
负责人：
Ronald Wayne Davis
金额：
$ 35.03万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-23 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10705040
关键词：
Address Area Behavior Biology Chemistry Communities Coupling DNA Development Dideoxy Chain Termination DNA Sequencing Electrodes Encapsulated Engravings Exposure to Failure Genome Glass Individual Label Lasers Length Light Liquid Chromatography Mass Chromatography Measurement Measures Medicine Methods Nanostructures Nucleotides Oils Oligonucleotides Optics Pattern Phase Physiologic pulse Polystyrenes Porosity Positioning Attribute Process Production Reaction Reagent Refuse Disposal Research Personnel Running Sampling Series Shapes Single-Stranded DNA Site Solid Spottings Surface Surface Tension System Technology Testing Transportation Visualization Withdrawal Workload base cost design electric field gene product gene synthesis laser tweezer nano nanophotonic nanoscale novel optic trap optic tweezer optical traps plasmonics qubit surface coating synthetic biology tripolyphosphate wasting

项目摘要

Abstract Long strand oligonucleotide synthesis continues to be limited by its diminishing returns, with a current maximum length of ~ 250 bases. As a general rule, one of every 100 molecules will fail to couple, meaning that the average synthesis run is said to have a coupling efficiency (CE) of 99%. The formula, CEn, where n is the number of bases added during synthesis, states the longer the strand generated, the more failure strands will be produced. For example, synthesis of a 40 base strand with a 99% CE will generate 68% full-length product (FLP) as opposed to synthesis of a 200 base strand, which will yield 13% FLP with the same CE. While there are other factors that may influence CE (i.e. synthesis parameters and quality of reagents), the main problem is inadequate accessibility of reagent to each of the molecules on the surface of the solid substrate (i.e. polystyrene beads or controlled-pore-glass). The most common case is when beads are packaged inside a column sandwiched between two porous filters; here, stacking of beads causes reduced surface area exposure to synthesis reagents, whereby DNA molecules become unreacted or only partially reacted. Moreover, spent reagents and unwanted byproducts become trapped within the support and carry over into consecutive cycles, further contaminating the synthesis run. To circumvent these limitations, we propose a novel method that allows us to control the actions of an individual bead through dielectrophoresis on a plasmonic surface. Here, reactions are tuned to completely encapsulate each bead with minimal volume reagent droplets for high-precision synthesis. Because each bead is isolated in solution, byproducts cannot become trapped, and each has maximum contact with all synthesis reagents; it is this intimate 1:1 ratio of bead to reagent that will significantly increase the base addition efficiency allowing the production of ultra-long strands of DNA > 1000 bases. Until very recently, far-field optics (i.e. optical tweezers) could not be applied at the nano-scale due to diffraction-limited focused spot size; therefore, researchers began studying effects of plasmonic nanostructures where light waves are concentrated directly onto the bead. In our platform, reagent droplets of precise volume and concentration are formed by pulsed laser cavitation; droplets are then transported along the plasmonic surface to encapsulate individual beads by overcoming surface tension barrier using dielectrophoretic forces generated by an AC electrical field. Thus, this approach of encapsulating a bead into a droplet and pulling it out can be employed for a large range of droplet and bead sizes with the appropriate electrode design. We believe the key to maximizing oligonucleotide purity and yield during synthesis lies in determining the minimal volume/concentration of each reagent necessary to coat the surface of an individual bead. With our proposed platform of synthesis on a plasmonic surface, we have the capability to address each individual bead for an accurate, optimized ratio of bead to reagent droplet of defined concentration. These developments are necessary to realize the full potential of synthetic biology, by making large-scale projects accessible to the entire community that will fuel discoveries in genome biology and medicine.

摘要长链寡核苷酸合成继续受到其收益递减的限制，目前的最大值为长度约250个碱基。一般来说，每100个分子中就有一个分子会失败，这意味着据说合成运行具有99%偶联效率（CE）。公式CEn，其中n是在合成过程中添加的碱基，指出产生的链越长，产生的失败链就越多。例如，具有99%CE的40个碱基的链的合成将产生68%的全长产物（FLP），与200个碱基的链的合成相反，这将产生具有相同CE的13%的FLP。虽然有其他可能影响CE的因素（即合成参数和试剂质量），主要问题是不充分试剂对固体基质（即聚苯乙烯珠或聚苯乙烯珠）表面上的每种分子的可接近性可控孔隙玻璃）。最常见的情况是当珠子被包装在一个柱夹在在两个多孔过滤器之间;这里，珠的堆叠导致暴露于合成试剂的表面积减少，由此DNA分子变得未反应或仅部分反应。此外，用过的试剂和不需要的副产物被捕获在载体内并进入连续的循环，进一步污染了载体。合成运行。为了规避这些限制，我们提出了一种新的方法，使我们能够控制的行动通过等离子体表面上的介电电泳分离单个珠。在这里，反应被调整到完全用最小体积的试剂液滴封装每个微珠，以实现高精度合成。因为每颗珠子在溶液中被隔离，副产物不会被捕获，并且每个副产物都与所有合成物有最大的接触。试剂;正是这种紧密的1：1的珠与试剂比例将显著提高碱添加效率允许产生> 1000个碱基的超长DNA链。直到最近，远场光学（即光学镊子）由于衍射限制的聚焦光斑尺寸而不能应用于纳米尺度;因此，研究人员开始研究等离子体纳米结构的效应，在珠上。在我们的平台中，通过脉冲激光形成精确体积和浓度的试剂液滴然后，液滴沿着等离子体表面传输，以通过以下方式封装单个珠粒：使用由AC电场产生的介电泳力克服表面张力屏障。因此，这将珠包封到液滴中并将其拉出的方法可用于大范围的液滴以及珠粒尺寸与适当的电极设计。我们相信最大化寡核苷酸纯度的关键和合成过程中的产率取决于确定每种试剂的最小体积/浓度，在单个珠粒的表面涂覆。通过我们提出的在等离子体表面上合成的平台，我们能够针对每个单独的珠粒，以获得珠粒与试剂液滴的准确、优化的比率，确定浓度。这些发展对于实现合成生物学的全部潜力是必要的，使整个社区都能接触到大规模的项目，这将推动基因组生物学的发现，药

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Microparticle electrical conductivity measurement using optoelectronic tweezers.

使用光电镊子测量微粒电导率。

DOI：
10.1063/5.0169565
发表时间：
2023
期刊：
Journal of applied physics
影响因子：
3.2
作者：
Ren,Wei;Zaman,MohammadAsif;Wu,Mo;Jensen,MichaelAnthony;Davis,RonaldWayne;Hesselink,Lambertus
通讯作者：
Hesselink,Lambertus

Dynamically controllable plasmonic tweezers using C-shaped nano-engravings.