Developmental Biology of the Normal Mouse Prostate

正常小鼠前列腺的发育生物学

• 批准号: 6892807
• 负责人: Susan Kasper
• 金额: $ 28.05万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-15 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6892807
• 关键词: androgens; biomarker; carcinogenesis; cell growth regulation; cell proliferation; gene expression; genetically modified animals; histogenesis; hormone regulation /control mechanism; laboratory mouse; male; molecular cloning; neoplasm /cancer genetics; prostate; prostate neoplasms; protein purification; protein structure function

DESCRIPTION (provided by applicant): The developing prostate has the highest rate of cell proliferation which subsequently ceases in the mature adult, resulting in a growth quiescent gland. We have focused on the morphogenesis of the mouse dorsal prostatic lobes and have identified proteins by mass spectrometry in the developing 4 week prostate that are absent in the mature 10 week prostate. These proteins will be characterized further and those which meet the criteria that they are: 1) expressed during normal prostate development in the 4 week old prostate, 2) absent in the 10 week growth quiescent prostate, 3) regulated by androgens, 4) continue to be expressed in the LPB-Tag model for prostate cancer and 5) epithelial cell specific will be utilized to develop novel models for prostate cancer. We have the composite probasin promoter (ARR2PB) which is prostate epithelial cell specific and which targets high levels of transgene expression to the mouse prostate. The transgenic mouse models will allow us to analyze the effects of over-expressing these endogenous proteins on the growth and differentiation of the normal prostate gland and to determine their role in the development of prostate cancer. Our HYPOTHESIS is that proteins that regulate normal prostate morphogenesis may promote the development of prostate cancer. The Specific Aims of this proposal are: Specific Aim I. To characterize the proteins previously identified in the growing 4 week prostate which are absent in the 10 week growth quiescent prostate. Specific Aim II. To define the expression of these proteins in the developing prostate and determine whether they are re-expressed in prostate cancer (using the LPB-Tag model for prostate cancer). Specific Aim III. To determine the function of these proteins in prostate tumorigenesis using the transgenic mouse model.

描述（由申请人提供）：发展中的前列腺具有最高的