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Regulation of plant cell-to-cell communication

植物细胞间通讯的调节

基本信息

批准号：
2454361
负责人：
金额：
--
依托单位：
University of St Andrews
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2020
资助国家：
英国
起止时间：
2020 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2454361
关键词：
Regulation plant cell communication

项目摘要

Plasmodesmata are membrane-lined cytoplasmic channels which are of fundamental importance to plant development, stress responses and defence against pathogens. They also serve as conduits for infection by plant viruses, major crop pathogens with a global agro-economic impact. Building on recent advances in understanding plasmodesmata architecture and virus transport by the Tilsner lab, and a novel method for identifying protein-protein interactions developed by the Hemsley lab, this project will characterize a contractile machinery at plasmodesmata putatively required for the regulation of intercellular communication in plants, and likely co-opted for virus transport.Several actin-associated proteins have been localized to plasmodesmata and in some cases shown to be involved in regulation of plant cell-cell connectivity and virus transport. The aims of the project are: Analyse interactions between actin-related proteins at plasmodesmata Identify additional components of the putative contractile structure Test effects of individual and combined knock-out mutations on plant development, cell-cell connectivity, virus transport and plasmodesmata ultrastructure.The PhD candidate will use a novel system for detection of protein-protein interactions in vivo to identify new components of a putative actin-related contractile system at plasmodesmata. The plasmodesmal localization of new candidates will be confirmed by fluorescence and electron microscopy. Co-immunoprecipitation and yeast-two-hybrid assays will be used to analyse reciprocal interactions between all previously known and newly identified components. Functional relations between these proteins will be probed using crosses of Arabidopsis knock out lines, which will be analysed for developmental phenotypes, virus susceptibility and intercellular molecular transport. The student will travel to a collaborating lab for ultrastructural (electron tomography) analysis of plasmodesmata morphology in mutant lines.

胞连丝是膜衬细胞质通道，对植物发育、应激反应和防御病原体至关重要。它们还充当植物病毒感染的渠道，植物病毒是对全球农业经济产生影响的主要作物病原体。基于蒂尔斯纳实验室在理解胞间连丝结构和病毒运输方面的最新进展，以及赫姆斯利实验室开发的一种识别蛋白质-蛋白质相互作用的新方法，该项目将描述胞间连丝的收缩机制，该机制被认为是调节植物细胞间通讯所必需的，并且可能被共同选择用于病毒运输。几种肌动蛋白相关蛋白已被定位于胞间连丝，在某些情况下显示参与植物细胞间连接和病毒运输的调节。该项目的目标是：分析胞间连丝肌动蛋白相关蛋白之间的相互作用识别假定的收缩结构的其他成分测试单个和组合敲除突变对植物发育、细胞间连接、病毒运输和胞间连丝超微结构的影响。博士候选人将使用一种新的系统来检测体内蛋白质-蛋白质相互作用，以识别假定的肌动蛋白相关的新成分胞间连丝的收缩系统。新候选物的胞间连丝定位将通过荧光和电子显微镜证实。免疫共沉淀和酵母双杂交测定将用于分析所有先前已知和新鉴定的成分之间的相互相互作用。这些蛋白质之间的功能关系将通过拟南芥敲除株系的杂交进行探索，并分析其发育表型、病毒敏感性和细胞间分子转运。学生将前往合作实验室对突变株系的胞间连丝形态进行超微结构（电子断层扫描）分析。