CORE-MOLECULAR BIOLOGY

分子生物学模块提供涉及转基因、敲除和敲入小鼠的生产以及用于一系列应用的基因构建体的构建的服务（例如，原位杂交探针）、DNA克隆和探针的制备以及聚合酶链反应引物设计和反应条件优化的咨询或直接服务。该模块还提供了有限规模的DNA 测序、磷光成像和流式细胞术的咨询或直接服务，用于细胞分析，以及共享分子生物学仪器的维护和监督。该模块占据了专为其专用而设计的新实验室。该模块由两名全职分子生物学技术人员组成，他们在分子克隆，DNA操作，畜牧业和显微注射方面具有先进的技能。该模块的服务通过使分子生物学成为Vision核心组的任何成员都可以使用的工具来增强研究环境。通过提供培训和支持， 使用分子生物学的现代共享仪器，包括STORM磷光成像仪和Beckman Coulter FC 500流式细胞仪。

The Molecular Biology Module provides services involved with the production of transgenic, knock-out and knock-in mice and construction of gene constructs used for an array of applications (e.g., in situ hybridization probes), preparation of DNA clones and probes, and consultation or direct service for the design of polymerase chain reaction primers and optimization of reaction conditions. The module also provides limited- scale DNA sequencing, consultation or direct services for phosphorimaging and flow cytometry for cell analysis, and maintenance and oversight of shared molecular biology instrumentation. The module occupies a new laboratory designed for its exclusive use. The module is staffed by two full-time molecular biology technicians with advanced skills in molecular cloning, DNA manipulations, animal husbandry and microinjection. The services of this module enhance the environment for research by making molecular biology an accessible tool for any member of the vision core group. The environment is further enhanced by providing training and support in the use of modern shared instrumentation for molecular biology, including the STORM phosphorimager and Beckman Coulter FC500 flow cytometer.