CORE-MOLECULAR BIOLOGY

核心分子生物学

• 批准号: 6949368
• 负责人: Thomas Almon Ferguson
• 金额: $ 18.66万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6949368
• 关键词: biomedical facility; biotechnology; flow cytometry; gene targeting; microinjections; molecular biology; nucleic acid probes; polymerase chain reaction; training; vision

The Molecular Biology Module provides services involved with the production of transgenic, knock-out and knock-in mice and construction of gene constructs used for an array of applications (e.g., in situ hybridization probes), preparation of DNA clones and probes, and consultation or direct service for the design of polymerase chain reaction primers and optimization of reaction conditions. The module also provides limited- scale DNA sequencing, consultation or direct services for phosphorimaging and flow cytometry for cell analysis, and maintenance and oversight of shared molecular biology instrumentation. The module occupies a new laboratory designed for its exclusive use. The module is staffed by two full-time molecular biology technicians with advanced skills in molecular cloning, DNA manipulations, animal husbandry and microinjection. The services of this module enhance the environment for research by making molecular biology an accessible tool for any member of the vision core group. The environment is further enhanced by providing training and support in the use of modern shared instrumentation for molecular biology, including the STORM phosphorimager and Beckman Coulter FC500 flow cytometer.

分子生物学模块提供的服务涉及转基因、敲除和敲入小鼠的生产和用于一系列应用的基因结构的构建(例如，原位杂交探针)、DNA克隆和探针的制备，以及聚合酶链式反应引物设计和反应条件优化的咨询或直接服务。该模块还提供有限规模的DNA 用于细胞分析的磷成像和流式细胞术的测序、咨询或直接服务，以及共享分子生物学仪器的维护和监督。该模块占据了一个专为其独家使用而设计的新实验室。该模块由两名全职分子生物学技术人员组成，他们在分子克隆、DNA操作、畜牧业和显微注射方面拥有先进技能。这一模块的服务使分子生物学成为VISION核心小组任何成员都可以使用的工具，从而改善了研究环境。通过提供培训和支持，环境得到了进一步改善 使用现代共享分子生物学仪器，包括风暴磷成像仪和Beckman Coulter FC500流式细胞仪。