Flow Cytometry Core

摘要 北达科他州流式细胞术和细胞分选(ND-FCCS)核心的建立是为了提供调查人员 和他们的实验室工作人员与这个Cobre，北达科他州IDEA网络的生物医学 卓越研究(INBRE)和达科他州癌症翻译活动合作(DaCCoTA) CTR以及北达科他州的研究人员，以下是：咨询设计中的 荧光细胞分析或分选实验，包括荧光选择和参数校准； 培训和监督收集流式细胞仪数据的仪器；流式细胞仪细胞分类； 咨询数据分析和分析软件的使用；以及维护、维护和何时 适当升级仪器和分析软件。在阶段1中，使用了核心 由所有持有Cobre项目的研究人员以及与 更大的传染病小组，新德里大学、南加州州立大学和米诺特州立大学ND INBRE的研究人员， 以及UND、NDSU和美国农业部人类营养中心的其他非相关调查人员。在阶段中 核心能够用BD交响乐取代过时的BD LSR2，资金来自这两个Cobre 和ND INBRE，后来又增加了第三台流式细胞仪，索尼MA900细胞分类器，资金来自 DaCCoTA CTR，以及增加Core持有的FlowJo分析软件的许可证数量 主要供HPI Cobre调查人员使用。核心将继续提供服务 进入第二阶段，并为那些希望核心执行 收集他们准备/染色的细胞的流式细胞仪数据，以及持续的荧光细胞分选 对于科布雷、INBRE和CTR的调查人员，以及北达科他州的非相关研究人员。 为确保持续的可持续发展，将在 COBRE为核心的所有用户提供的第二阶段。

Summary The North Dakota Flow Cytometry and Cell Sorting (ND-FCCS) Core was established to provide investigators and their laboratory staff associated with this COBRE, the North Dakota IDeA Networks of Biomedical Research Excellence (INBRE), and the Dakota Cancer Collaborative on Translational Activity (DaCCoTA) CTR, as well as researchers across the state of North Dakota, the following: consultation in the design of fluorescent cell analysis or sorting experiments including fluorochrome selection and parameter calibration; training and oversight on the instrumentation to collect flow cytometry data; flow cytometric cell sorting; consultation on data analysis and usage of analysis software; as well as maintenance, upkeep, and when appropriate, upgrading of the instrumentation and analysis software. During Phase 1, the Core was utilized heavily by all of the researchers holding COBRE projects as well as most of the laboratories associated with the larger infectious disease group, investigators in the ND INBRE at UND, NDSU, and Minot State University, and other non-associated investigators at UND, NDSU, and the USDA Human Nutrition Center. During Phase 1 the Core was able to replace the obsolete BD LSR2 with a BD Symphony with funds from both this COBRE and the ND INBRE, and later to add a third flow cytometer, a Sony MA900 Cell Sorter, with funds from the DaCCoTA CTR, as well as to increase the number of licenses for FlowJo analytical software held by the Core for use predominately by the HPI COBRE investigators. The Core will continue with the services provided moving into Phase 2, as well as initiating full service for those investigators wanting the Core to perform the collection of flow cytometric data on their prepared/stained cells, as well as continued fluorescent cell sorting for the COBRE, INBRE, and CTR investigators, as well as non-associated researchers across North Dakota. To ensure ongoing sustainability, a fee for service or instrument usage fee will be implemented during the Phase 2 period of the COBRE for all users of the Core.