Flow Cytometry Core

总结 建立了北达科他州流式细胞术和细胞分选（ND-FCCS）中心，为研究者提供 以及他们与COBRE相关的实验室工作人员，北达科他州IDEA生物医学网络 卓越研究（INBRE）和达科他州癌症转化活动协作（DaCCoTA） CTR，以及整个北达科他州的研究人员，以下： 荧光细胞分析或分选实验，包括荧光染料选择和参数校准; 对收集流式细胞术数据的仪器进行培训和监督;流式细胞术细胞分选; 咨询数据分析和分析软件的使用;以及维护，保养，当 适当时，升级仪器和分析软件。在第1阶段， 主要是由所有持有COBRE项目的研究人员以及与COBRE项目相关的大多数实验室进行的。 更大的传染病组，UND，NDSU和迈诺特州立大学的ND INBRE的研究人员， 以及UND、NDSU和USDA人类营养中心的其他非相关研究人员。在阶段 1.核心能够用BD Symphony取代过时的BD LSR 2，资金来自COBRE 和ND INBRE，后来增加了第三台流式细胞仪，索尼MA 900细胞分选仪，资金来自 DaCCoTA CTR，以及增加核心持有的FlowJo分析软件的许可证数量 主要由HPI COBRE调查人员使用。核心小组将继续提供服务 进入第二阶段，以及为那些希望核心执行 收集其制备/染色细胞的流式细胞术数据，以及持续的荧光细胞分选 COBRE、INBRE和CTR研究人员以及北达科他州的非相关研究人员。 为了确保持续的可持续性，在服务期间将收取服务费或仪器使用费。 COBRE第二阶段适用于所有核心用户。

Summary The North Dakota Flow Cytometry and Cell Sorting (ND-FCCS) Core was established to provide investigators and their laboratory staff associated with this COBRE, the North Dakota IDeA Networks of Biomedical Research Excellence (INBRE), and the Dakota Cancer Collaborative on Translational Activity (DaCCoTA) CTR, as well as researchers across the state of North Dakota, the following: consultation in the design of fluorescent cell analysis or sorting experiments including fluorochrome selection and parameter calibration; training and oversight on the instrumentation to collect flow cytometry data; flow cytometric cell sorting; consultation on data analysis and usage of analysis software; as well as maintenance, upkeep, and when appropriate, upgrading of the instrumentation and analysis software. During Phase 1, the Core was utilized heavily by all of the researchers holding COBRE projects as well as most of the laboratories associated with the larger infectious disease group, investigators in the ND INBRE at UND, NDSU, and Minot State University, and other non-associated investigators at UND, NDSU, and the USDA Human Nutrition Center. During Phase 1 the Core was able to replace the obsolete BD LSR2 with a BD Symphony with funds from both this COBRE and the ND INBRE, and later to add a third flow cytometer, a Sony MA900 Cell Sorter, with funds from the DaCCoTA CTR, as well as to increase the number of licenses for FlowJo analytical software held by the Core for use predominately by the HPI COBRE investigators. The Core will continue with the services provided moving into Phase 2, as well as initiating full service for those investigators wanting the Core to perform the collection of flow cytometric data on their prepared/stained cells, as well as continued fluorescent cell sorting for the COBRE, INBRE, and CTR investigators, as well as non-associated researchers across North Dakota. To ensure ongoing sustainability, a fee for service or instrument usage fee will be implemented during the Phase 2 period of the COBRE for all users of the Core.