Flow Cytometry Core

流式细胞术核心

• 批准号: 10462728
• 负责人: David S. Bradley
• 金额: $ 21.64万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-13 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10462728
• 关键词: Advisory Committees; Applications Grants; Biomedical Research; Biotechnology; Calibration; Cell Separation; Cells; Centers of Research Excellence; Collection; Communicable Diseases; Computer software; Consultations; Data; Data Analyses; Educational workshop; Ensure; Experimental Designs; Fee-for-Service Plans; Fees; Financial compensation; Floor; Flow Cytometry; Fluorochrome; Funding; Human; Immunology; Institution; Instruction; Laboratories; Licensing; Maintenance; Malignant Neoplasms; Microbiology; North Dakota; Pathology; Periodicity; Phase; Research Personnel; Schedule; Services; Site; Software Design; Sorting - Cell Movement; Stains; Training; United States Department of Agriculture; Universities; Update; cell preparation; design; experimental study; instrument; instrumentation; medical schools; member; nutrition; operation; pathogen; webinar

Summary The North Dakota Flow Cytometry and Cell Sorting (ND-FCCS) Core was established to provide investigators and their laboratory staff associated with this COBRE, the North Dakota IDeA Networks of Biomedical Research Excellence (INBRE), and the Dakota Cancer Collaborative on Translational Activity (DaCCoTA) CTR, as well as researchers across the state of North Dakota, the following: consultation in the design of fluorescent cell analysis or sorting experiments including fluorochrome selection and parameter calibration; training and oversight on the instrumentation to collect flow cytometry data; flow cytometric cell sorting; consultation on data analysis and usage of analysis software; as well as maintenance, upkeep, and when appropriate, upgrading of the instrumentation and analysis software. During Phase 1, the Core was utilized heavily by all of the researchers holding COBRE projects as well as most of the laboratories associated with the larger infectious disease group, investigators in the ND INBRE at UND, NDSU, and Minot State University, and other non-associated investigators at UND, NDSU, and the USDA Human Nutrition Center. During Phase 1 the Core was able to replace the obsolete BD LSR2 with a BD Symphony with funds from both this COBRE and the ND INBRE, and later to add a third flow cytometer, a Sony MA900 Cell Sorter, with funds from the DaCCoTA CTR, as well as to increase the number of licenses for FlowJo analytical software held by the Core for use predominately by the HPI COBRE investigators. The Core will continue with the services provided moving into Phase 2, as well as initiating full service for those investigators wanting the Core to perform the collection of flow cytometric data on their prepared/stained cells, as well as continued fluorescent cell sorting for the COBRE, INBRE, and CTR investigators, as well as non-associated researchers across North Dakota. To ensure ongoing sustainability, a fee for service or instrument usage fee will be implemented during the Phase 2 period of the COBRE for all users of the Core.

概括 建立了北达科他州流式细胞仪和细胞分选（ND-FCCS）核心以提供研究人员 及其实验室人员与此山脉，北达科他州的北达科他州思想网络有关 卓越研究（INBRE）和达科他州癌症转化活动合作（DACCOTA） CTR，以及北达科他州的研究人员，以下内容：咨询 荧光细胞分析或分类实验，包括荧光体选择和参数校准； 培训和监督仪器以收集流式细胞仪数据；流式细胞仪分类； 关于数据分析和使用分析软件的咨询；以及维护，维护以及何时 适当的，升级仪器和分析软件。在第1阶段，使用了核心 所有持有毛to项目的研究人员以及与大多数与之相关的实验室的研究人员 更大的传染病组，NDSU的ND INBRE的研究人员，NDSU和Minot州立大学， 以及UND，NDSU和USDA人类营养中心的其他非相关研究人员。在阶段 1核心能够用BD Symphony替换过时的BD LSR2，并用这两种毛绒的资金代替了 和ND INBRE，后来添加第三个流式细胞仪，Sony MA900细胞分道机，并带有来自 DACCOTA CTR，以及增加核心持有的FlowJo分析软件的许可证数量 主要由HPI COBRE研究人员使用。核心将继续提供提供的服务 进入第2阶段，并为那些希望核心执行的调查人员提供全面服务 收集其准备/染色细胞上的流式细胞仪数据，以及持续的荧光细胞分类 对于北达科他州的毛病，ybre和CTR研究人员以及与非相关的研究人员。 为了确保持续的可持续性，将在服务期间实施服务或工具使用费 核心所有用户的COBRE的第2阶段。