Interrogating the RAS-RAF-MEK-ERK pathway using Protein Interference.

使用蛋白质干扰询问 RAS-RAF-MEK-ERK 通路。

• 批准号: 2488489
• 金额: --
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2488489
• 关键词: Interrogating; RAS; RAF; MEK; ERK

This multi-disciplinary project will employ synthetic biology screens to identify novel regulators/inhibitors of the RAS-RAF-MEK-ERK pathway, a critical cell fate signalling pathway, using PhoreMost's protein interference (PROTEIN-i) screening technology. The ERK pathway normally promotes cell survival through ERK-catalysed phosphorylation of the pro-apoptotic protein BIMEL. The short form of BIM (BIMS) lacks these phosphorylation sites. This provides an opportunity to harness BIMS as an ERK-driven toxic gene, providing phenotypic selection for PROTEIN-I probes.

这个多学科项目将采用合成生物学筛选，以确定新的调节剂/抑制剂的RAS-RAF-MEK-ERK途径，一个关键的细胞命运信号通路，使用PhoreMost的蛋白质干扰（PROTEIN-i）筛选技术。ERK途径通常通过ERK催化的促凋亡蛋白BIMEL的磷酸化促进细胞存活。短形式的BIM（BIM）缺乏这些磷酸化位点。这提供了利用BIMS作为ERK驱动的毒性基因的机会，为蛋白质-I探针提供表型选择。