Glucose Signal Transduction Pathways in Plants

描述（由申请人提供）：葡萄糖是大多数生物体首选的通用营养素，在能量供应、碳储存、生物合成以及碳骨架和细胞壁形成中发挥重要作用。感知葡萄糖和葡萄糖缺乏的能力在各种生物体中具有根本的重要性，如大肠杆菌。大肠杆菌、酵母、苍蝇、哺乳动物和植物。葡萄糖作为多细胞动物和植物中的直接和中心信号分子的重要性直到最近才被认识到。在植物中，其生命围绕通过光合作用产生糖，葡萄糖已成为许多重要过程的关键调节剂，包括胚胎发生，萌发，幼苗发育，根，茎和芽生长，碳和氮代谢，繁殖，应激反应和衰老。我们的目标是了解植物如何感知葡萄糖信号来控制基因表达并调节生长和发育。对植物葡萄糖反应的研究将有助于我们更全面地了解从酵母到哺乳动物等真核生物中保守的葡萄糖信号传导机制。互补的基因组学，蛋白质组学，遗传学，分子，生物化学和细胞的策略和工具将被用来研究葡萄糖信号通路在拟南芥作为一个植物模型。四个具体目标是： 目标1。葡萄糖应答基因的全基因组鉴定和分子遗传学分析。我们将联合收割机结合葡萄糖信号突变体和全球基因表达谱的力量，以确定葡萄糖响应基因，并进行选定的靶基因的功能分析。 目标2.葡萄糖信号传导中HXK 1蛋白复合物的蛋白质组学和遗传学分析。 我们将确定核HXK 1蛋白复合物的关键组分，并使用遗传，细胞和生物化学工具研究葡萄糖信号转导中新型调控基因的功能。 目标3。gin 2抑制（g/s）突变体的遗传分析。 将采取遗传学方法来鉴定参与HXK 1介导的信号传导的新基因。 目标4。葡萄糖剥夺反应的基因组和反向遗传分析。 我们将研究拟南芥蛋白激酶（KIN 10和KIN 11）的功能，这些蛋白激酶控制葡萄糖剥夺和胁迫反应中的不同基因。

DESCRIPTION (provided by applicant): Glucose is a universal nutrient preferred by most organisms and serves essential roles in energy supply, carbon storage, biosyntheses, and carbon skeleton and cell wall formation. The ability to sense glucose and its deprivation is of fundamental importance in organisms as diverse as E. coli, yeast, flies, mammals, and plants. The significance of glucose as a direct and central signaling molecule in multi-cellular animals and plants has only been recently recognized. In plants whose life revolves around sugar production through photosynthesis, glucose has emerged as a key regulator of many vital processes, including embryogenesis, germination, seedling development, root, stem and shoot growth, carbon and nitrogen metabolism, reproduction, stress responses, and senescence. Our goal is to understand how plants sense glucose signals to control gene expression and modulate growth and development. Studies of glucose responses in plants will enhance our general understanding of glucose signaling mechanisms conserved in other eukaryotes from yeast to mammals. Complementary genomic, proteomic, genetic, molecular, biochemical, and cellular strategies and tools will be used to study glucose signaling pathways in Arabidopsis thaliana as a plant model. Four specific aims are: Aim 1. Genome-wide identification and molecular genetic analysis of glucose responsive genes. We will combine the power of glucose signaling mutants and global gene expression profiling to identify glucose responsive genes and perform functional analysis of selected target genes. Aim 2. Proteomic and genetic analysis of the HXK1 protein complexes in glucose signaling. We will identify the key components of the nuclear HXK1 protein complexes and investigate the function of novel regulatory genes in glucose signaling using genetic, cellular and biochemical tools. Aim 3. Genetic analysis of the gin2 suppressor (g/s) mutants. A genetic approach will be taken to identify novel genes involved in HXK1-mediated signaling. Aim 4. Genomic and reverse genetic analysis of glucose deprivation responses. We will study the functions of Arabidopsis protein kinases (KIN10 and KIN11) that control diverse genes in glucose deprivation and stress responses.