Glucose Signal Transduction Pathways in Plants

描述（由申请人提供）：葡萄糖是大多数生物体首选的普遍营养素，在能量供应，碳储存，生物合成，碳骨架和细胞壁形成中起着重要作用。在大肠杆菌、酵母、苍蝇、哺乳动物和植物等多种生物体中，感知葡萄糖及其剥夺的能力至关重要。葡萄糖作为多细胞动物和植物的直接中枢信号分子的重要性直到最近才被认识到。在通过光合作用产生糖的植物中，葡萄糖已成为许多重要过程的关键调节器，包括胚胎发生，发芽，幼苗发育，根，茎和芽生长，碳和氮代谢，繁殖，应激反应和衰老。我们的目标是了解植物如何感知葡萄糖信号来控制基因表达和调节生长发育。植物中葡萄糖反应的研究将增强我们对从酵母到哺乳动物等其他真核生物中保守的葡萄糖信号传导机制的总体理解。互补的基因组学、蛋白质组学、遗传学、分子学、生化学和细胞策略和工具将用于研究拟南芥作为植物模型的葡萄糖信号通路。四个具体目标是：

DESCRIPTION (provided by applicant): Glucose is a universal nutrient preferred by most organisms and serves essential roles in energy supply, carbon storage, biosyntheses, and carbon skeleton and cell wall formation. The ability to sense glucose and its deprivation is of fundamental importance in organisms as diverse as E. coli, yeast, flies, mammals, and plants. The significance of glucose as a direct and central signaling molecule in multi-cellular animals and plants has only been recently recognized. In plants whose life revolves around sugar production through photosynthesis, glucose has emerged as a key regulator of many vital processes, including embryogenesis, germination, seedling development, root, stem and shoot growth, carbon and nitrogen metabolism, reproduction, stress responses, and senescence. Our goal is to understand how plants sense glucose signals to control gene expression and modulate growth and development. Studies of glucose responses in plants will enhance our general understanding of glucose signaling mechanisms conserved in other eukaryotes from yeast to mammals. Complementary genomic, proteomic, genetic, molecular, biochemical, and cellular strategies and tools will be used to study glucose signaling pathways in Arabidopsis thaliana as a plant model. Four specific aims are: Aim 1. Genome-wide identification and molecular genetic analysis of glucose responsive genes. We will combine the power of glucose signaling mutants and global gene expression profiling to identify glucose responsive genes and perform functional analysis of selected target genes. Aim 2. Proteomic and genetic analysis of the HXK1 protein complexes in glucose signaling. We will identify the key components of the nuclear HXK1 protein complexes and investigate the function of novel regulatory genes in glucose signaling using genetic, cellular and biochemical tools. Aim 3. Genetic analysis of the gin2 suppressor (g/s) mutants. A genetic approach will be taken to identify novel genes involved in HXK1-mediated signaling. Aim 4. Genomic and reverse genetic analysis of glucose deprivation responses. We will study the functions of Arabidopsis protein kinases (KIN10 and KIN11) that control diverse genes in glucose deprivation and stress responses.