Plant Nutrient-Growth Signaling Network

植物养分生长信号网络

• 批准号: 10734306
• 负责人: JEN SHEEN
• 金额: $ 40.42万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10734306
• 关键词: Agriculture; Amino Acids; Animal Diseases; Animals; Arabidopsis; Architecture; Biochemical; Biology; Biomass; Calmodulin; Carbon; Cell Nucleus; Complex; Coupled; Coupling; Cytoplasm; Development; Developmental Process; Ecosystem; Environmental Protection; Gene Expression; Gene Expression Regulation; Genes; Genetic; Genetic Screening; Genetic Transcription; Genomics; Goals; Growth; Growth Factor; Health; Human; Knowledge; Lateral; Life; Link; Longevity; Mediating; Metabolic; Metabolism; Methodology; Molecular; Mouse-ear Cress; Nitrates; Nitrogen; Nodule; Nucleic Acids; Nutrient; Organ; Organism; Phosphorylation; Phosphotransferases; Physiological; Planets; Plant Roots; Plant Shoots; Plants; Play; Productivity; Protein Kinase; Proteins; Regulation; Reiterated Genes; Research; Research Project Grants; Role; Shapes; Signal Transduction; Signaling Molecule; Sirolimus; Somatotropin; Subgroup; System; Systems Analysis; Transcription Coactivator; Transcriptional Regulation; Transducers; Transgenic Organisms; biological systems; chemical genetics; combinatorial; experimental study; functional genomics; genetic analysis; genome-wide; innovation; mutant; novel; nutrition; organ growth; plant growth/development; programs; protein complex; response; sensor; success; transcription factor; transcriptional reprogramming; transcriptome

Nutrient signaling integrates and coordinates gene expression, metabolism, and growth. In multicellular organisms, growth factors and hormones are ineffective in growth promotion without the support of nutrient signaling networks. However, surprisingly little is known about the primary nutrient signaling mechanisms in plants and animals. Plants play a central role in bridging the conversion of inorganic nitrogen to organic nitrogen in the global nitrogen cycle by assimilating inorganic nitrate to generate amino acids, nucleic acids, and organic nitrogen-carbon molecules, which are essential to build and sustain lives from plants to humans. Despite the fundamental and multifaceted regulatory roles of nitrate in gene expression, metabolism, growth, and development, the molecular and cellular mechanisms of nitrate signaling remain largely elusive in multicellular plants. Hampered by gene redundancy and mutant lethality, classical genetic screens had limited success in identifying key nitrate signaling components in plants over the past two decades. By taking integrated molecular, cellular, biochemical, functional genomic, chemical genetic, and systems analyses, we have discovered a surprising molecular link between specific Ca2+-sensor protein kinases (CPKs) and the NODULE INCEPTION-LIKE PROTEIN (NLP) transcription factors as the primary regulators of the nitrate-signaling network in plants. Our research has demonstrated the unique role of nitrate as a central signaling molecule in transcriptome reprogramming and shoot-root coordination to shape organ biomass and architecture. We also recently discovered the first plant nitrate sensor NLP7 with a dual function as a transcription activator, and the combinatorial actions of multiple NLPs in controlling the primary nitrate responses (PNR) central to coordinate plant root and shoot development. We propose to build on our new findings and innovative experimental platforms to elucidate the molecular and cellular basis of the nutrient-growth network that orchestrates system-wide transcription and modulates plant developmental processes. We will integrate complementary strategies and methodologies to advance our understanding of nutrient signaling mechanisms for three specific aims: Aim1. Elucidate the function and action of the NLP7 nitrate sensor complex Aim 2. Dissect the intracellular Ca2+ signaling mechanism triggered by nitrate Aim 3. Uncover the CPK-TOR link in nitrate signaling The proposed research to unravel the nitrate signaling mechanisms will establish new paradigms in the action of nutrient sensor complexes, nutrient-mediated Ca2+ signaling, as well as transcriptional and developmental regulation with sustained scientific impact beyond plant biology.

营养信号整合和协调基因表达、代谢和生长。在

项目成果

Glucose-driven TOR-FIE-PRC2 signalling controls plant development.

• DOI: 10.1038/s41586-022-05171-5
• 发表时间: 2022-09
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Ye, Ruiqiang;Wang, Meiyue;Du, Hao;Chhajed, Shweta;Koh, Jin;Liu, Kun-hsiang;Shin, Jinwoo;Wu, Yue;Shi, Lin;Xu, Lin;Chen, Sixue;Zhang, Yijing;Sheen, Jen
• 通讯作者: Sheen, Jen

Dynamic Nutrient Signaling Networks in Plants.

• DOI: 10.1146/annurev-cellbio-010521-015047
• 发表时间: 2021-10-06
• 期刊: Annual review of cell and developmental biology
• 影响因子: 11.3
• 作者: Li L;Liu KH;Sheen J
• 通讯作者: Sheen J

Model-driven discovery of calcium-related protein-phosphatase inhibition in plant guard cell signaling

• DOI: 10.1371/journal.pcbi.1007429
• 发表时间: 2019-10-01
• 期刊: PLOS COMPUTATIONAL BIOLOGY
• 影响因子: 4.3
• 作者: Maheshwari, Parul;Du, Hao;Albert, Reka
• 通讯作者: Albert, Reka

Integration of nutrient, energy, light, and hormone signalling via TOR in plants.

• DOI: 10.1093/jxb/erz028
• 发表时间: 2019-02
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Yue Wu;Lin Shi;Lei Li;Liwen Fu;Yanlin Liu;Yan Xiong;J. Sheen

TOR and RPS6 transmit light signals to enhance protein translation in deetiolating Arabidopsis seedlings.

• DOI: 10.1073/pnas.1809526115
• 发表时间: 2018-12-11
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Chen GH;Liu MJ;Xiong Y;Sheen J;Wu SH
• 通讯作者: Wu SH