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Plant Nutrient-Growth Signaling Network

植物养分生长信号网络

基本信息

批准号：
10734306
负责人：
JEN SHEEN
金额：
$ 40.42万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-01 至 2027-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10734306
关键词：
Agriculture Amino Acids Animal Diseases Animals Arabidopsis Architecture Biochemical Biology Biomass Calmodulin Carbon Cell Nucleus Complex Coupled Coupling Cytoplasm Development Developmental Process Ecosystem Environmental Protection Gene Expression Gene Expression Regulation Genes Genetic Genetic Screening Genetic Transcription Genomics Goals Growth Growth Factor Health Human Knowledge Lateral Life Link Longevity Mediating Metabolic Metabolism Methodology Molecular Mouse-ear Cress Nitrates Nitrogen Nodule Nucleic Acids Nutrient Organ Organism Phosphorylation Phosphotransferases Physiological Planets Plant Roots Plant Shoots Plants Play Productivity Protein Kinase Proteins Regulation Reiterated Genes Research Research Project Grants Role Shapes Signal Transduction Signaling Molecule Sirolimus Somatotropin Subgroup System Systems Analysis Transcription Coactivator Transcriptional Regulation Transducers Transgenic Organisms biological systems chemical genetics combinatorial experimental study functional genomics genetic analysis genome-wide innovation mutant novel nutrition organ growth plant growth/development programs protein complex response sensor success transcription factor transcriptional reprogramming transcriptome

项目摘要

Nutrient signaling integrates and coordinates gene expression, metabolism, and growth. In multicellular organisms, growth factors and hormones are ineffective in growth promotion without the support of nutrient signaling networks. However, surprisingly little is known about the primary nutrient signaling mechanisms in plants and animals. Plants play a central role in bridging the conversion of inorganic nitrogen to organic nitrogen in the global nitrogen cycle by assimilating inorganic nitrate to generate amino acids, nucleic acids, and organic nitrogen-carbon molecules, which are essential to build and sustain lives from plants to humans. Despite the fundamental and multifaceted regulatory roles of nitrate in gene expression, metabolism, growth, and development, the molecular and cellular mechanisms of nitrate signaling remain largely elusive in multicellular plants. Hampered by gene redundancy and mutant lethality, classical genetic screens had limited success in identifying key nitrate signaling components in plants over the past two decades. By taking integrated molecular, cellular, biochemical, functional genomic, chemical genetic, and systems analyses, we have discovered a surprising molecular link between specific Ca2+-sensor protein kinases (CPKs) and the NODULE INCEPTION-LIKE PROTEIN (NLP) transcription factors as the primary regulators of the nitrate-signaling network in plants. Our research has demonstrated the unique role of nitrate as a central signaling molecule in transcriptome reprogramming and shoot-root coordination to shape organ biomass and architecture. We also recently discovered the first plant nitrate sensor NLP7 with a dual function as a transcription activator, and the combinatorial actions of multiple NLPs in controlling the primary nitrate responses (PNR) central to coordinate plant root and shoot development. We propose to build on our new findings and innovative experimental platforms to elucidate the molecular and cellular basis of the nutrient-growth network that orchestrates system-wide transcription and modulates plant developmental processes. We will integrate complementary strategies and methodologies to advance our understanding of nutrient signaling mechanisms for three specific aims: Aim1. Elucidate the function and action of the NLP7 nitrate sensor complex Aim 2. Dissect the intracellular Ca2+ signaling mechanism triggered by nitrate Aim 3. Uncover the CPK-TOR link in nitrate signaling The proposed research to unravel the nitrate signaling mechanisms will establish new paradigms in the action of nutrient sensor complexes, nutrient-mediated Ca2+ signaling, as well as transcriptional and developmental regulation with sustained scientific impact beyond plant biology.

营养信号整合和协调基因表达，代谢和生长。在多细胞生物、生长因子和激素在生长促进中是无效的，营养信号网络的支持。然而，令人惊讶的是，植物和动物的营养信号机制。植物在连接在全球氮循环中通过同化作用将无机氮转化为有机氮无机硝酸盐生成氨基酸、核酸和有机氮碳分子，从植物到人类，它们对建立和维持生命至关重要。尽管基本的和硝酸盐在基因表达、代谢、生长和发育中的多方面调节作用，硝酸盐信号传导的分子和细胞机制在多细胞中仍然很难理解，植物由于基因冗余和突变体致死性的限制，传统的遗传筛选方法在过去的二十年里，成功地确定了植物中关键的硝酸盐信号成分。通过采用整合的分子、细胞、生物化学、功能基因组学、化学遗传学和系统分析，我们发现了一个令人惊讶的分子之间的联系，具体的钙离子传感器蛋白激酶（CPKs）和脑啡肽诱导样蛋白（NLP）转录因子作为植物中硝酸盐信号网络的主要调节剂。我们的研究表明硝酸盐作为转录组重编程中的中心信号分子的独特作用，茎-根协调形成器官生物量和结构。我们最近还发现具有作为转录激活因子的双重功能的第一植物硝酸盐传感器NLP 7，以及组合的多个NLP在控制初级硝酸盐响应（PNR）中的作用植物根和芽的发育。我们建议在新发现的基础上，阐明营养生长网络的分子和细胞基础的实验平台协调系统范围的转录并调节植物发育过程。我们将整合互补策略和方法以增进我们对营养素的了解三个具体目标的信号机制：目标1.阐明NLP 7硝酸盐传感器复合物的功能和作用目标2.探讨硝酸盐引发的细胞内Ca2+信号转导机制目标3.发现硝酸盐信号中的CPK-TOR联系这项旨在揭示硝酸盐信号传导机制的研究将为人类建立新的范式。营养传感器复合物的作用、营养介导的Ca 2+信号传导以及转录和发育调控，具有超越植物生物学的持续科学影响。

项目成果

期刊论文数量（20）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Glucose-driven TOR-FIE-PRC2 signalling controls plant development.

DOI：
10.1038/s41586-022-05171-5
发表时间：
2022-09
期刊：
NATURE
影响因子：
64.8
作者：
Ye, Ruiqiang;Wang, Meiyue;Du, Hao;Chhajed, Shweta;Koh, Jin;Liu, Kun-hsiang;Shin, Jinwoo;Wu, Yue;Shi, Lin;Xu, Lin;Chen, Sixue;Zhang, Yijing;Sheen, Jen
通讯作者：
Sheen, Jen

Dynamic Nutrient Signaling Networks in Plants.