Nanoscale dissection of the Ephrin-Eph Receptor signalling axis in cellular invasion

辅助性细胞串扰在发育过程中起着至关重要的作用，使领导细胞和跟随细胞在血管生成和神经元迁移过程中能够相互沟通。调节这一过程的一个关键轴是EPhin-Eph受体信号。肾上腺素配体在表达Eph受体酪氨酸激酶的相邻细胞中诱导正向信号传递，但也在表达EPhin的细胞内表现出反向信号。与许多发育途径一样，这种信号在疾病中重现，例如在胰腺癌侵袭中。我们将在基于2D/3D细胞的模型中使用基因工程和超分辨率显微镜来询问这种反向信号的动力学，从而为这一信号轴的调节和功能提供重要的见解。

Juxtacrine cellular cross-talk is critical during development, allowing leader and follower cells to communicate during angiogenesis and neuronal migration. One key axis mediating this process is Ephrin-Eph receptor signalling. Ephrin ligands induce forward signalling in neighbouring cells expressing Eph receptor tyrosine kinases, but also exhibit reverse signalling within the Ephrin-expressing cell. As with many developmental pathways, this signalling is recapitulated in disease, for example in pancreatic cancer invasion. We will use genetic engineering and super-resolution microscopy, in 2D/3D cell-based models to interrogate the dynamics of this reverse signalling, providing crucial insights into the regulation and function of this signalling axis.