Nanoscale dissection of the Ephrin-Eph Receptor signalling axis in cellular invasion

近端细胞串扰在发育过程中至关重要，它允许领导细胞和跟随细胞在血管生成和神经元迁移过程中进行通信。介导这一过程的一个关键轴是Ephrin-Eph受体信号传导。Ephrin配体在表达Eph受体酪氨酸激酶的邻近细胞中诱导正向信号传导，但在表达Ephrin的细胞中也表现出反向信号传导。与许多发育途径一样，这种信号传导在疾病中重现，例如在胰腺癌侵袭中。我们将使用基因工程和超分辨率显微镜，在2D/3D细胞模型来询问这种反向信号的动力学，提供对这种信号轴的调节和功能的重要见解。

Juxtacrine cellular cross-talk is critical during development, allowing leader and follower cells to communicate during angiogenesis and neuronal migration. One key axis mediating this process is Ephrin-Eph receptor signalling. Ephrin ligands induce forward signalling in neighbouring cells expressing Eph receptor tyrosine kinases, but also exhibit reverse signalling within the Ephrin-expressing cell. As with many developmental pathways, this signalling is recapitulated in disease, for example in pancreatic cancer invasion. We will use genetic engineering and super-resolution microscopy, in 2D/3D cell-based models to interrogate the dynamics of this reverse signalling, providing crucial insights into the regulation and function of this signalling axis.