An in vivo genetic screen of lung function candidate genes using tissue-specific RNAi in Drosophila melanogastor.

使用黑腹果蝇组织特异性 RNAi 对肺功能候选基因进行体内遗传筛选。

• 批准号: 2569267
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2569267
• 关键词: vivo; genetic; screen; lung; function

To highlight and characterise the candidate genes for Chronic Obstructive Pulmonary Disorder (COPD) using the trachea and dorsal thorax of Drosophila melanogastor as a model system. Two RNAi screens of 61 lung function gene orthologues will be carried out in succession on the fly dorsal thorax and tracheal system respectively. 'Hits' from these screens are to be carefully analysed and the extent of interplay between candidate gene orthologues and the maintenance of epithelial cell homeostasis determined. A final shortlist of the three most relevant and therapeutically amenable genes will be translated into human systems for expression studies.

以果蝇气管和背胸为模型系统，筛选慢性阻塞性肺疾病（COPD）的候选基因。分别在果蝇背胸和气管系统上进行61个肺功能基因直向同源物的两次RNAi筛选。从这些筛选的“命中”要仔细分析，并确定候选基因直系同源物之间的相互作用程度和上皮细胞稳态的维持。最终的三个最相关和治疗上最合适的基因的候选名单将被翻译到人类系统中进行表达研究。