Magnetic nanoswitches for sensing biomolecules

用于传感生物分子的磁性纳米开关

• 批准号: 6945181
• 负责人: RALPH WEISSLEDER, MD, PHD
• 金额: $ 39.38万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6945181
• 关键词: bioassay; bioengineering /biomedical engineering; biosensor device; biotechnology; chemical genetics; clinical research; high throughput technology; human tissue; magnetic field; nanomedicine; nanotechnology; neoplasm /cancer diagnosis; nuclear magnetic resonance spectroscopy; particle; protein quantitation /detection; rapid diagnosis; small molecule; technology /technique development; telomerase; telomere; virus

DESCRIPTION (provided by applicant): Robust, versatile, and high-throughput biosensors are required for genomic and proteomic research applications, for high throughput screening in drug development, for rapid point of care assays and, potentially for in vivo imaging applications. In particular, there is a need for assay methodologies that a) are highly sensitive, b) minimize sample preparation and allow measurements in turbid/obscure media, c) measure widely different types of molecules using the same format and instrument and, d) can be run in single tube point of care or high throughput screening formats. We have developed a novel magnetic nanosensor technology that uses "magnetic relaxation switches" (MRSW), i.e. magnetic nanoparticles that are induced to switch between dispersed and clustered states by analytes, with changes in the spin-spin relaxation time (T2) of adjacent water molecules. The broad goals of this application are to optimize and extend MRSW methodology, and to develop MRSW assays for key areas of biomedical research and needs. Specific aim 1 involves the optimization of nanoparticles for MRSW assays, and improvements and extensions of the MR based instrumentation the method uses. In Specific Aim 2 we will apply the MRSW methodology to key issues of cancer biology (telomerase and telomere length), microbiology (virus detection and characterization), and panomics.

描述（由申请人提供）：基因组和蛋白质组学研究应用所需的鲁棒，多功能和高通量生物传感器，用于药物开发中的高吞吐量，用于快速的护理测定点以及可能在体内成像应用中进行。特别是，需要测定方法，即a）高度敏感，b）最小化样品制备并允许在浑浊/模糊的介质中进行测量，c）使用相同的格式和仪器测量大量不同类型的分子，并且可以在单管的护理点或高吞吐量筛选格式中运行。我们已经开发了一种新型的磁性纳米传感器技术，该技术使用“磁性弛豫开关”（MRSW），即通过分析物诱导在分散状态和聚集状态之间切换的磁性纳米颗粒，并且邻近水分子的自旋旋转弛豫时间（T2）的变化发生了变化。 该应用的广泛目标是优化和扩展MRSW方法，并为生物医学研究和需求的关键领域开发MRSW分析。特定目标1涉及对MRSW分析的纳米颗粒的优化，以及该方法使用的基于MR的仪器的改进和扩展。在特定目标2中，我们将将MRSW方法论应用于癌症生物学（端粒酶和端粒长度），微生物学（病毒检测和表征）和Panomics的关键问题。