Development of Objective Methods for Fluorosis Diagnosis

氟中毒诊断客观方法的发展

• 批准号: 6914697
• 负责人: ESPERANZA A MARTINEZ-MIER
• 金额: $ 15.15万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6914697
• 关键词: adolescence (12-20); bioimaging /biomedical imaging; clinical research; dental visualization; diagnosis design /evaluation; digital imaging; fluorimetry; fluorosis; human subject; image processing; middle childhood (6-11); photography; therapy adverse effect

DESCRIPTION: Methods currently used to diagnose Dental fluorosis rely on subjective measurements based on epidemiological indices. This has resulted in absence of diagnostic sensitivity, specificity and has led to inconsistencies in data summary and report. The proposed investigation Aims at developing objective and quantitative methods for fluorosis diagnosis using imaging technology (Quantitative Light Fluorescence [QLF] and digital intraoral photography) and visual examination. Validation of results will be provided by direct microanalysis of fluoride content, histological examination and 3-d quantification of mineral content of fluoride-related hypomineralizations. Both extracted teeth and children ages 7 to 13 will be examined as part of the study. Identification of selected surfaces as fluorosis cases, caries lesions or sound/non-fluorosis cases will be performed visually and will be recorded with QLF and digital intraoral photographs. Quantification of QLF images will be conducted. Methods to quantify photographic images using image analysis software will be developed. A method to quantify fluoride-related hypomineralizations using micro CT will be also developed. Sensitivity, specificity and repeatability of each method will be assessed. The development of objective methods for Dental fluorosis diagnosis will improve Dental fluorosis and fluoride research. The proposed investigation will provide the opportunity to identify such methods for the assessment of Dental fluorosis. It will provide the methodological framework to resolve discrepancies that currently make it difficult to compare results of studies dealing with the epidemiology, and the biological and esthetic effects of Dental fluorosis.

描述：目前用于诊断氟斑牙的方法依赖于基于流行病学指数的主观测量。这导致缺乏诊断灵敏度和特异性，并导致数据总结和报告不一致。本研究旨在通过影像学技术（定量光荧光[QLF]和数字口内摄影）和目视检查，建立客观、定量的氟中毒诊断方法。通过氟含量的直接微量分析、组织学检查和氟相关低矿化的矿物质含量的三维定量，将提供结果的验证。作为研究的一部分，将对拔牙和7至13岁的儿童进行检查。将通过视觉识别选定的表面为氟中毒病例、龋齿病变或健全/非氟中毒病例，并使用QLF和数字口内照片进行记录。将对QLF图像进行量化。将开发使用图像分析软件量化摄影图像的方法。还将开发一种使用微型CT定量氟相关矿化不足的方法。将评估每种方法的灵敏度、特异性和重复性。氟斑牙客观诊断方法的发展将促进氟斑牙和氟研究的发展。拟议的调查将提供机会，以确定这种方法来评估氟斑牙。它将提供一个方法框架，以解决目前难以比较流行病学研究结果的差异，以及氟斑牙的生物和美学影响。